FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217993
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2550.11697623318179941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTCT200.00749617951.494788
CACGCTT350.001293562239.7448444
TGTACGG350.001372303939.270164
GTATCAA10150.038.6552351
CGTGCAA556.603668E-638.2404857
GGTATCA4300.036.817891
TTAACGG601.3364714E-534.50403635
TTGTACG400.002646383834.35353
CGGCAAA750.00307016933.31596470
TATCAAC12050.032.80812
AACCGTG652.117779E-532.25906854
TAACCGT652.1279546E-532.23647753
AACGCAG12150.032.2107246
ATCAACG12250.031.6893523
TCAACGC12150.031.6746984
CAACGCA12300.031.2597055
ACGCAGA12900.030.6041347
GCTTGTA1600.030.1146341
CGCAGAG13350.029.572538
ACGGGTT703.9685E-529.425595
Produced by FastQC (version 0.11.5)