FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences870067
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATAGTAAATTACCGCTCACTTTCGGCGGAGGGA33660.38686675853698627No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32180.36985657426382107No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA25000.2873341938034657No Hit
GTCAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTA21470.24676260563841634No Hit
GTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGAGA20130.23136149285055058No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19270.22147719658371137No Hit
GTCTGGGACAGACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATT18790.2159603800626848No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18720.21515584432003515No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18660.21446624225490682No Hit
GTGTATTACTGTGCGAGACTTGGCGGTTATTACGATGTTTTGACTGCTTA18250.20975396147652997No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17420.20021446624225492No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16810.19320351191345034No Hit
GTCTCCAGCCACCCTGTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGACTGTGACCAG16600.19078990468550122No Hit
GTCTTTGTATCCAGGGGAAAGAGCCACCCTCTCCTGCGGGGCCAGTCAGA16190.1860776239071244No Hit
GTGTATTACTGTCAGCAGTATAGTAAATTACCGCTCACTTTCGGCGGAGGGACCAAGGTTCAGATCAAACGAA15840.18205494519387586No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15400.17699786338293488No Hit
GTGCAGCCACAGTTCGTTTGATCTGAACCTTGGTCCCTCCGCCGAAAGTG15140.17400958776737885No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14730.169297306989002No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14350.16492982724318933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13160.15125271961814435No Hit
GTATAGTAAATTACCGCTCACTTTCGGCGGAGGGACCAAGGTTCAGATCAAACGAACTGTGGCTGCACCAT12960.14895404606771662No Hit
GTCCCAGACCCACTGCCACGGAACCTGTCTGGGATGCCAGTGGCCCTGGT12840.14757484193746No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGA12810.14723004090489583No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12500.14366709690173285No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12450.14309242851412593No Hit
CTCCTGCGGGGCCAGTCAGACTGTGACCAGCAGCTATTTAGCCTGGTACC12340.14182815806139068No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT12300.14136842335130512No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTACCGGAGAAATTGTGTTGACGCAGTCTC12230.14056388760865543No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12140.13952948451096295No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12110.1391846834783988No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11960.137460678315578No Hit
AACCTGGCCTGGCGCCCAGGCTCCTCATCTATGAGACATCCACCAGGGCC11890.1366561425729283No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC11870.13642627521788553No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG11250.12930038721155956No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11140.12803611675882431No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.12608224424096076No Hit
GACTTCACTCTCACCATCAACAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATAGTAAATTA10620.12205956552771224No Hit
CCTATTACACCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGGCCAACAACCAGTTCTCCCTGAAG10550.12125502978506252No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10180.11700248371677123No Hit
GGATACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATA10100.11608301429660015No Hit
TTACTATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGTTGATGGTGAGAGTGAAGTCTGTC10000.1149336775213863No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9960.11447394281130074No Hit
GCACAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGAGACATCCACCAGGGCCACTGGCATCCCAGACAGGTT9940.11424407545625798No Hit
GCTCAGTCAGGACCCAGAGGGAACCATGGATACCCCAGCGCAGCTTCTCT9880.11355447339112966No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9830.11297980500352273No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9800.11263500397095856No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG9650.11091099880813776No Hit
ATTACACCCCGTCCCTCAAGAGTCGAGTCACCATATCCGTAGACACGGCC9520.10941686100035974No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGAACCTTGGTCC9510.10930192732283835No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9260.10642858538480371No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9260.10642858538480371No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9060.10412991183437598No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8960.10298057505916211No Hit
GTCAGACTGTGACCAGCAGCTATTTAGCCTGGTACCAGCACAAACCTGGC8870.10194617196146963No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8860.10183123828394824No Hit
CAGTAATACACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCTTCAGGGA8830.10148643725138408No Hit
GTACCAGCACAAACCTGGCCTGGCGCCCAGGCTCCTCATCTATGAGACATCCACCAGGGCCACTGGCATCCCAGA8730.10033710047617021No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8710.10010723312112746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAA150.002276254869.59688650
CGAACTA353.3314063E-759.0261329
GTGGTAT2700.053.5053181
CCTCGTT607.9805194E-443.85455770
GGTATCA10550.043.688161
GTATCAA29150.042.59711
TACGGAT6650.042.53562570
TGGTATC3500.042.258282
CGTACTA1750.041.31829529
CGTATGC1500.039.2510141
TATCAAC32350.038.2771532
CTTATAC2000.037.8359031
GCGAACT557.5109638E-637.54911828
AGCGAAC557.525803E-637.5383227
ACCGTAC1850.037.2001327
ATAGACG7250.036.29342370
ACTAGAT1900.036.28797532
ACGAGAC2000.036.10986322
GTACTAG2100.036.08398430
ATCAACG34450.035.7441673
Produced by FastQC (version 0.11.5)