FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512153
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.1341134131268463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.041.3896681
GTATCAA67700.040.4353871
GTGGTAT8550.040.172221
TATCAAC80250.034.2402462
TGGTATC9850.034.1728942
TCAACGC82000.033.4257354
ATCAACG82400.033.3051573
CAACGCA84300.032.554515
AACGCAG86800.031.775166
ACGCAGA92250.029.890027
CGCAGAG92850.029.585928
AGAGTAC96100.028.76401511
CAGAGTA98150.028.23321210
GCAGAGT98450.027.9379049
AGTACGG61850.026.1491913
GAGTACG62750.025.82886312
ACGTAGA4350.023.70971327
GTACGGG71000.022.63416914
ACGGGTC2800.022.07745216
AGTACTT51850.021.92079413
Produced by FastQC (version 0.11.5)