FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512153
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34750.22980478827208622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9950.044.1557921
GTATCAA74300.040.9765741
GGTATCA30300.039.5351681
TGGTATC11250.039.033942
TATCAAC84700.035.9678542
ATCAACG89150.034.1476023
TCAACGC89400.033.9495664
CAACGCA90450.033.695815
AACGCAG93100.032.773476
ACGCAGA99850.030.4893467
CGCAGAG102000.029.846688
AGAGTAC104250.029.26820411
CAGAGTA105950.028.70162210
GCAGAGT107250.028.2898629
AGTACGG66250.025.84768313
GAGTACG67550.025.60290112
ACGGGTA3750.023.75247416
GAGTACT56850.023.01208712
TACGGGC16550.022.97463815
AGTACTT58100.022.34089513
Produced by FastQC (version 0.11.5)