FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838087
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTTATACAGCTCCAACAAAAAGAACTACCTAGCTTGGTATCAGCAGAA135650.737995535575846No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT103840.5649351744503933No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC71810.39067791676890157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70460.3833333242659352No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG65070.35400935864298044No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT63180.34372692913882746No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG62270.338776129747939No Hit
AACCAGGACAGTCTCCCAAGTTGCTCATGCACTGGGCTTCTATCCGGGAA60540.32936417046635985No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC55030.2993873521764748No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTGGATGGTGGCCCTCTCGCCCAGAGACGCAGCCAGGGAGTCTGGAGA54500.2965039195641991No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT53750.29242359039588445No Hit
GTGCATGAGCAACTTGGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTA51840.2820323521139097No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC48490.2638068818287709No Hit
ATAATAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA48440.2635348598842166No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT46640.2537420698802614No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG46290.2518379162683812No Hit
GTCTCCAGACTCCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATA45470.2473767563776905No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC45060.24514617643234515No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG44480.24199072187551512No Hit
ATATTATAATAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT43400.23611504787314203No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAGGTAGTTCTTTTTGTTGGAGCTG42420.23078341775987754No Hit
CTCCCAAGTTGCTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCT41150.223874060368198No Hit
GTTTATTACTGTCAGCAATATTATAATAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA40920.22262275942324822No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC40640.22109943653374403No Hit
ACTTGGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTAGGTAGTTCTTTTTGTTGGAGCTGTATAAAACACT39600.21544138008701436No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG39480.21478852742008403No Hit
GTCAGGGACCCCGGATTCCCGGATAGAAGCCCAGTGCATGAGCAACTTGGGAGACTGTCCTGGTTTCTGCTGATA39090.21266675625256043No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37790.2055941856941483No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT37740.20532216374959403No Hit
ATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAAAAAGAACTACCTAGCTTGGTATCAGCAGAAA37340.20314598819315952No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG37310.20298277502642692No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36410.19808638002444934No Hit
ACCTAGCTTGGTATCAGCAGAAACCAGGACAGTCTCCCAAGTTGCTCATG36380.19792316685771671No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35810.1948221166897976No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG35140.19117702263276984No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATATTATAATAAC34100.18551896618604016No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA33720.1834515994074274No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33550.18252672479594273No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT33220.18073137996188426No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATATTATAATAACCCGCTCACTTT33050.17980650535039963No Hit
GTCTCCCAAGTTGCTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT32610.1774127122383217No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATATTATAATAACCCGC31290.17023133290208786No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT31140.16941526706842494No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA31020.1687624144014946No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30920.16821837051238597No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30880.16800075295674255No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC30710.16707587834525786No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT29670.16141782189852819No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG29480.1603841385092218No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA29150.15858879367516335No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC28770.1565214268965506No Hit
CCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGC28190.1533659723397206No Hit
CTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAG27840.1514618187278404No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC27680.15059134850526662No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27130.14759910711516921No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT26700.14525971839200214No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG26630.1448788876696261No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC26410.14368199111358712No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC26060.14177783750170694No Hit
CTCCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA26000.14145141116824175No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA25910.140961771668044No Hit
GGTTATTATAATATTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT25830.14052653655675712No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG25720.13992808827873762No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAAAAAGAACTACC25460.1385135741670552No Hit
GCTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT25340.13786072150012485No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTG25240.13731667761101624No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA25080.13644620738844246No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT24800.1349228844989383No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA24630.13399800988745364No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24320.1323114738312169No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG24150.13138659921973225No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGATAGAAGCCCAGTGCAT24030.1307337465528019No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG24020.13067934216389104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.12959125438567382No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA23770.12931923244111948No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT23720.12904721049656517No Hit
GGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGG23250.12649020421775467No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC22840.12425962427240932No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT22690.12344355843874637No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGTTATT22640.12317153649419206No Hit
TTATAATATTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC22170.12061453021538153No Hit
TTATTACTGTCAGCAATATTATAATAACCCGCTCACTTTCGGCGGAGGGA21870.11898239854805567No Hit
ACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAAAAA21780.11849275904785792No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT21580.11740467126964066No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC21440.1166430098248886No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA21050.11452123865736497No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA20860.11348755526805858No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC20700.11261708504548479No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20680.11250827626766308No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20660.11239946748984134No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT20630.11223625432310876No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC20620.11218184993419789No Hit
CTTGGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTAGGTAGTTCTTTT20150.10962484365538737No Hit
TAGAAGCCCAGTGCATGAGCAACTTGGGAGACTGTCCTGGTTTCTGCTGA20130.10951603487756563No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA20110.10940722609974392No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20000.10880877782172443No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT19940.10848235148825927No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA19760.10750307248786374No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19520.10619736715400305No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19170.10429321354212288No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC19150.10418440476430114No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCG19100.10391238281974684No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC18960.10315072137499477No Hit
ATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC18890.10276989065261873No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA18470.10048490631836252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6700.055.945511
GTATCAA59100.039.218051
TATCAAC66600.034.6973882
AGCCTAT2600.034.45133632
ATCAACG70200.033.113973
TCAACGC70400.032.9710544
GTAGTTT2950.032.96891470
TATCTCG1700.032.47170636
CAACGCA72600.032.114035
AACGCAG72450.032.0855876
CGTATGC2050.030.3766641
ACGAGAC2400.030.0883222
CGCAGAG78700.029.4841758
ACGCAGA78950.029.4794987
AGAGTAC80400.028.90350711
CAGAGTA81000.028.73185210
TCGTATG2050.028.67415240
GCAGAGT81700.028.4856769
CTTATAC2850.027.7521441
TCGAGTA657.7421014E-427.05193362
Produced by FastQC (version 0.11.5)