FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1838087
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT97840.5322925411038759No Hit
GTTTTATACAGCTCCAACAAAAAGAACTACCTAGCTTGGTATCAGCAGAA96530.525165566156553No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT65950.3587969448671363No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG63060.34307407647189714No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC59360.3229444525748781No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC58990.3209314901851762No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG55050.2994961609542965No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT52800.28725517344935253No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49590.2697913646089657No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC49460.2690841075531245No Hit
GTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA45710.24868246171155117No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT45440.24721354321095793No Hit
ATAATAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA45270.24628866859947324No Hit
AACCAGGACAGTCTCCCAAGTTGCTCATGCACTGGGCTTCTATCCGGGAA44920.24438451498759306No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC43840.23850884098521996No Hit
ATATTATAATAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT42750.232578762593936No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCAC41460.22556059642443474No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT40670.22126264970047665No Hit
GTGCATGAGCAACTTGGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTA40220.21881445219948784No Hit
GTTTATTACTGTCAGCAATATTATAATAACCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA40110.21821600392146834No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGAT39660.21576780642047957No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG39290.21375484403077763No Hit
GTCTCCAGACTCCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATA38870.2114698596965214No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38800.21108902897414542No Hit
GGTTATTATAATATTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAAT38750.2108170070295911No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC37550.2042884803602876No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACC37020.2014050477480119No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA36690.19960970291395347No Hit
TTATAATATTGCTGACAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTC35990.19580139569019311No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG35710.19427807280068898No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35410.1926459411333631No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA35130.19112261824385895No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG34910.18992572168782No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG32940.17920805707238016No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATATTATAATAAC31720.17257072162525494No Hit
GTCAGGGACCCCGGATTCCCGGATAGAAGCCCAGTGCATGAGCAACTTGGGAGACTGTCCTGGTTTCTGCTGATA31510.17142822945812683No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30770.16740230467872305No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG30660.16680385640070355No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA30610.16653183445614925No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT30320.16495410717773423No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATATTATAATAACCCGCTCACTTT30190.16424685012189302No Hit
GGCTGAAGATGTGGCGGTTTATTACTGTCAGCAATATTATAATAACCCGC29980.1631043579547649No Hit
CTCCCAAGTTGCTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCT29870.16250590967674544No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA29870.16250590967674544No Hit
CAGTAATAAACCGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCA29740.16179865262090423No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT29580.16092818239833043No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCGGGTTATT29440.16016652095357836No Hit
GTCCTGGTTTCTGCTGATACCAAGCTAGGTAGTTCTTTTTGTTGGAGCTG29200.15886081561971768No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT28730.15630380934090715No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28480.1549436996181356No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28430.15467167767358128No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC28140.15309395039516627No Hit
ACTTGGGAGACTGTCCTGGTTTCTGCTGATACCAAGCTAGGTAGTTCTTTTTGTTGGAGCTGTATAAAACACT28040.15254990650605765No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC27590.15010170900506886No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG26660.14504210083635866No Hit
ATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAAAAAGAACTACCTAGCTTGGTATCAGCAGAAA26530.14433484378051747No Hit
GTATAAAACACTCTGGCTGGACTTGCAGTGGATGGTGGCCCTCTCGCCCAGAGACGCAGCCAGGGAGTCTGGAGA26490.14411722622487402No Hit
GAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCG26320.14319235161338936No Hit
GGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTT25930.1410705804458657No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG25650.1395472575563616No Hit
ACCTAGCTTGGTATCAGCAGAAACCAGGACAGTCTCCCAAGTTGCTCATG25060.13633739861062072No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC24900.13546692838804691No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA24610.1338892011096319No Hit
GTCTCCCAAGTTGCTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGT24090.13106017288626706No Hit
GACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCT24090.13106017288626706No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC23760.12926482805220862No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC23520.12795912271834795No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA23490.12779590955161535No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA22950.1248580725504288No Hit
ACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCA22530.12257308821617258No Hit
ACCTTGGTCCCTCCGCCGAAAGTGAGCGGGTTATTATAATATTGCTGACAGTAATAAACCGCCACATCTTCAGC22480.12230106627161826No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22430.12202904432706396No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC22190.12072333899320327No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22170.12061453021538153No Hit
CTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA22090.12017929510409464No Hit
GCTGATGGTGAGAGTGAAATCTGTCCCAGACCCGCTGCCACTGAATCGGT21850.11887358977023393No Hit
TTATTACTGTCAGCAATATTATAATAACCCGCTCACTTTCGGCGGAGGGA21840.11881918538132308No Hit
CCTTGGTCCCTCCGCCGAAAGTGAGCGGGTTATTATAATATTGCTGACAGTAATAAACCGCCACATCTTCAGC21620.11762228882528411No Hit
CTCCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAA21340.11609896593577998No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21170.1151740913242953No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC20910.1137595772126129No Hit
CCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTGCAAGTCCAGC20850.11343315087914772No Hit
CTGCAAGTCCAGCCAGAGTGTTTTATACAGCTCCAACAAAAAGAACTACC20410.11103935776706979No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20370.11082174021142634No Hit
CTACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGCGTCTCTGGGCGAGAGGGCCACCATCCACTG20340.11065852704469374No Hit
CTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAG20190.10984246121103082No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA20170.1097336524332091No Hit
GCTCATGCACTGGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGATTT20160.10967924804429824No Hit
GGGCTTCTATCCGGGAATCCGGGGTCCCTGACCGATTCAGTGGCAGCGGG20120.10946163048865477No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19990.10875437343281356No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19510.10614296276509219No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19500.10608855837618134No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT19400.10554451448707271No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC18910.10287869943044045No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT18900.1028242950415296No Hit
GACCGATTCAGTGGCAGCGGGTCTGGGACAGATTTCACTCTCACCATCAG18800.10228025115242097No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18760.10206263359677753No Hit
GAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGAT18700.10173620726331234No Hit
CCACTGAATCGGTCAGGGACCCCGGATTCCCGGATAGAAGCCCAGTGCAT18670.10157299409657976No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT18550.10092014142964942No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)