FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546571
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38890.15271516089675097No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGGCTGCTATCCCATGTT38170.14988782955590085No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37980.1491417282298432No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37870.14870977483054665No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG36680.14403682441997495No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACGGCACTCAGG34110.13394482227277385No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32840.12895772393544103No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.1264052720305069No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC27390.10755639642483952No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25990.10205880770651986No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC25640.10068441052693995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT14700.044.265241
TGGTATC15050.043.2366682
GGTATCA38500.041.1353761
GTATCAA95450.039.712731
TATCAAC106800.035.4607852
ATCAACG113000.033.5760843
TCAACGC114950.033.0664064
CAACGCA116900.032.6326375
AACGCAG120000.031.7322446
GCGTATA450.00467088230.603131
ACGCAGA128950.029.5182177
AGAGTAC128050.029.5095111
CGCAGAG129350.029.399758
CAGAGTA130900.028.94589210
GCAGAGT133850.028.4107829
TAAGGCG4050.027.21775229
GACTAAG3700.026.98557726
AGTACGG87000.026.7007713
GAGTACG88400.026.31683512
CGATTGT1851.2998442E-524.73353470
Produced by FastQC (version 0.11.5)