FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2520533
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61820.24526558469974408No Hit
GTTACAATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA42750.16960698391967097No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG42150.16722653502255277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC41130.16317977189745186No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40890.16222759233860456No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39610.15714930135808577No Hit
GTATTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAG39500.15671288572694742No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37310.14802424725246605No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC35490.14080355226454086No Hit
CATCTATGCTGCATCCAGTTTACAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTAGATCTGGGACGGATTTCA35460.14068452981968496No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG34140.13544754224602495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33270.13199589134520356No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32810.13017088052407963No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32370.12842521799952628No Hit
GTCGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGACCATTAATAGCTATTTAAATTGGTATCAACAGAAA29400.11664199595879125No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG29170.11572949054822927No Hit
ACTTACTACTGTCAACAGAGTTACAATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA28350.11247621038883442No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCTCTTGCCGGGCAAGTCAGACCATTAATAG27970.1109685927539929No Hit
GAGTTACAATACCCTCCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT26880.10664411059089487No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT26540.10529518954919455No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGATTGTTGATGGTGAGAGTGAAATCCGTCCCAGATCTACTGCCA26530.10525551540090926No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT26260.10418431339720606No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26060.1033908304315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11450.042.0486221
CTAGCGC4450.034.8003428
ATCAACG106750.033.2139133
TCAACGC107450.033.093554
TAGCGCT4900.033.0221529
CAACGCA109600.032.5071145
GTATCAA113650.031.6210351
AACGCAG113000.031.5594586
ACTAGCG4950.030.58385827
TCGTATG2700.029.47456640
CGCAGAG122200.029.1437538
ACGCAGA122350.029.0805027
GGTATCA59850.028.5575851
AGAGTAC124800.028.48093411
GCAGAGT127750.027.8501639
CAGAGTA131250.027.13368210
TATCAAC132750.026.99372
ACGAGAC6100.026.49861122
CGTATGC3650.025.61489341
AGTACGG84050.025.35725613
Produced by FastQC (version 0.11.5)