FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206257
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47020.3898008467515629No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA31850.26403991852482517No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31730.2630451056449828No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31120.2579881401724508No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATT30880.2559985144127661No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29780.24687939634754452No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29080.24107632121513076No Hit
CTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT28620.23726287184240177No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27510.22806085270385998No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAG26050.2159572959991113No Hit
CTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGCGTAGCAACTGGCTCACTTTCGG25460.21106613267321972No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG24670.20451694788092423No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24300.20144960816807694No Hit
GCCTAGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGCGTAGCAAC23150.19191598473625437No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCTCACTTTCGGCGGAGGGAC23070.1912527761496928No Hit
GACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGCGTAGCAACTGG22980.19050666648981104No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22870.1895947546832889No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21590.17898341729830375No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT20460.1696155960131216No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA19280.15983326936133843No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19150.15875555540817585No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18340.15204056846923997No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17230.14283854933069817No Hit
TCTATGATGCATCCATCAGGGCCACTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT17130.1420095385974962No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16290.13504584843859974No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15580.1291598722328658No Hit
CATCTATGATGCATCCATCAGGGCCACTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCA15430.12791635613306285No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.1246832142735752No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14720.12203037992732892No Hit
GTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG14220.1178853262613191No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14190.11763662304135852No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14150.11730501874807774No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13980.11589570050163439No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13850.11481798654847185No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT13690.1134915693753487No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13680.11340866830202852No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13670.11332576722870831No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13430.1113361414690236No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13160.1090978124893783No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13130.1088491092694177No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC13090.10851750497613694No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTG12930.10719108780301378No Hit
GCGTAGCAACTGGCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTT12890.106859483509733No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12770.10586467062989063No Hit
GTGTTAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCC12720.10545016526328968No Hit
GGGTACCAGTGGCCCTGATGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGG12630.1047040556034079No Hit
GAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC12590.10437245131012711No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12520.10379214379688574No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12410.10288023199036359No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.044.9519961
GTATCAA54750.040.8612141
CGTATGC3350.036.15698241
TATCAAC62500.035.7394372
GCGTAGT3550.034.85897429
GACGCGT3550.034.85174626
CGCGTAG3650.033.90112328
ACGCGTA3650.033.89690427
ATCAACG66250.033.716453
TCAACGC66500.033.58974
AGACGCG3800.033.4618925
TCGTATG3450.033.0764840
CAACGCA67900.032.947745
AATCTCG2850.032.66182336
AACGCAG69200.032.3784376
TATGCCG3700.031.86260243
TATACAC4800.031.501113
TACACAT4400.031.2407675
CGAGACG4100.031.00703223
ACGAGAC4100.031.00574722
Produced by FastQC (version 0.11.5)