FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206257
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45760.37935531151321816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29070.24099342014181055No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28690.23784317935564314No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27570.22855825914378114No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27350.22673443553073683No Hit
GTCTGGGACAGACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATT26300.21802982283211622No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25800.2138847691661064No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23190.19224758902953518No Hit
CTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGCGTAGCAACTGGCTCACTTTCGG22390.18561550316391948No Hit
CTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT22260.18453778921075692No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22070.18296266881767317No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22030.1826310645243924No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCTCACTTTCGGCGGAGGGAC21370.17715959368525944No Hit
GCCTAGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGCGTAGCAAC19650.1629006090741857No Hit
GACTTCACTCTCTCCATCAGCAGCCTAGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGCGTAGCAACTGG19550.16207159834098372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19370.1605793790212202No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18200.15087995344275723No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18160.15054834914947643No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17970.1489732287563927No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17510.14515977938366367No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17320.14358465899057996No Hit
GTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCCCTCCGCCGAAAGTG16500.13678677097832384No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16500.13678677097832384No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15640.12965727867278698No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15590.129242773306186No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTGCACCTTGGTCC15540.12882826793958502No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15250.1264241368132993No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15040.1246832142735752No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14980.12418580783365402No Hit
CTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTACCAGCAAAAACCTGGCCAGGCTCCCAGGCT14290.11846563377456047No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14270.1182998316279201No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAG14060.11655890908819595No Hit
GTAATAAACAGCAAAATCTTCAGGCTCTAGGCTGCTGATGGAGAGAGTGAAGTCTGTCCCAGACCCACTGCCG14040.11639310694155558No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13910.11531539298839301No Hit
GTCCCAGACCCACTGCCGCTGAACCTGGCTGGGGTACCAGTGGCCCTGAT13780.11423767903523047No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13710.1136573715219891No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTTAGCAGCTACTTAGCCTGGTA13690.1134915693753487No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13430.1113361414690236No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCGCTGAACCTGGCTGGGGTACCA13170.10918071356269848No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13130.1088491092694177No Hit
GGGTACCAGTGGCCCTGATGGATGCATCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTTTGCTGGTACCAGG12470.10337763843028475No Hit
TCTATGATGCATCCATCAGGGCCACTGGTACCCCAGCCAGGTTCAGCGGCAGTGGGTCTGGGACAGACTTCACT12350.10238282555044241No Hit
GCGTAGCAACTGGCTCACTTTCGGCGGAGGGACCAAGGTGCAGATCAAACGAACTGTGGCTGCACCATCTGTCTT12070.1000615954974769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.044.554631
GTATCAA54050.042.509281
TATCAAC59750.038.0885432
ATCAACG63200.036.099973
TCAACGC63600.035.7294354
CAACGCA64900.034.9934235
AACGCAG65900.034.564946
CGCAGAG70600.032.3123938
ACGCAGA71750.031.7467547
GTGGTAT8800.031.2219961
AGAGTAC73000.031.20314411
CGATTGT850.004535134430.78465370
TGGTATC8500.030.691172
GCAGAGT75650.030.2912249
CAGAGTA77050.029.5630110
ACGGGGC3750.028.33349816
AGTACGG51750.025.95059613
GAGTACT36050.025.84206612
GAGTACG52700.025.6064412
AGTACTT36150.025.3031513
Produced by FastQC (version 0.11.5)