FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502064
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGTTGGATCCG8760.17447974760189935No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT8100.16133401319353707No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7500.14938334554957136No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7220.14380636731572072No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC7070.1408187004047293No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6540.13026227731922624No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC6440.1282704993785653No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT6300.12548201026163996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6200.123490232320979No Hit
GTAATAAACGGCCGTGTCTGCGGCCGTCACAGAGTTCAGGTTCAGGGAGA6180.1230918767328468No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT5700.11353134261767424No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5690.11333216482360814No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT5600.11153956467701329No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA5340.10636094203129481No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5330.10616176423722873No Hit
GTTCTCTGGGGTCCCAGACAGATTCAGTGGCAGTGGGGCAGGGACAGATT5260.10476751967876605No Hit
ATGTATGACAGTGGGAGCACCTACTACAACCGGTCCCTCAAGAGTCGAGC5230.10416998629656778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAGC200.00711625452.1865651
TCCGATG200.00732503151.80636236
GTGGTAT2650.048.1453971
GGTATCA8100.044.2738461
TGGTATC2800.043.103142
CTACGGT400.001794744437.19145668
TGTTAGA1901.2732926E-1136.94528670
GTATCAA17600.036.637671
TACGGTG500.003086267333.2996469
GTGCTAC652.3269848E-531.8300111
TATCAAC20800.031.1668872
TTCGGGG5350.031.01269570
ATCAACG21100.030.7237553
TCAACGC21300.030.435274
CAACGCA21500.030.1521515
CTTAGGC500.005356176829.75316468
AACGCAG22300.028.9158296
AACCTTG900.00676303128.36203870
GCTATAG500.007749983627.586013
TATACTG500.007749983627.586015
Produced by FastQC (version 0.11.5)