FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences502064
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT9490.1890197265687243No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9180.18284521495267536No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT7310.14559896746231557No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC7180.14300965613945632No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGTTGGATCCG7050.14042034481659707No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6900.13743267790560565No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT6630.1320548774658211No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC6530.13006309952516013No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5650.11253545364734376No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5570.11094203129481502No Hit
GTATCAACGCAGAGTACGGGAATTAGGACTCCTCAGGTCACCTTCTCACA5440.10835271997195577No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC5420.10795436438382358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6050.049.0160521
TGGTATC1850.046.5650562
GTGGTAT2000.044.8268931
GTATCAA16250.040.7420731
TATCAAC19050.034.3676952
ATCAACG19900.032.697213
TCAACGC19950.032.4200674
CCGGAGT4600.031.34887370
CAACGCA21000.031.1143135
GTCTTAC450.00463188830.6508641
AACGCAG21450.030.4615656
TACGGGT1401.4551915E-1129.48846415
AGAGTAC23200.028.4602811
CGCAGAG23100.028.2857388
TTCGGGG3950.028.2103870
CGAACCT755.7462134E-527.88090553
ACAAACG755.8698417E-527.79394348
GCAGAGT23650.027.627939
ACGCAGA24000.027.3683137
CGTTGAT906.9612997E-626.8007722
Produced by FastQC (version 0.11.5)