FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1659596
Sequences flagged as poor quality0
Sequence length20-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.12725988734607702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25450.042.8799481
GTGGTAT8550.040.6666151
GTATCAA65850.040.6207541
TGGTATC9000.038.634452
TATCAAC72850.036.5769272
TCAACGC77950.034.2721564
ATCAACG78400.034.1193543
CAACGCA80250.033.41865
AACGCAG81500.032.9060446
ACGCAGA87450.030.658847
CGCAGAG88200.030.320098
AGAGTAC89800.029.47236311
CAGAGTA91450.029.01587310
GCAGAGT92750.028.8318219
AGTACGG61000.024.65565713
GAGTACG61700.024.48749412
TCGTATG2300.024.07528340
AGTACTT48300.023.37177513
GAGTACT49250.023.06070912
CGTCTAA1253.2210573E-622.29584150
Produced by FastQC (version 0.11.5)