FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726404
Sequences flagged as poor quality0
Sequence length20-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15400.2120032378676329No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7790.10724059889538054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38750.042.1417271
GTGGTAT5750.038.1846851
TATCAAC44800.036.4356352
GGTATCA14900.036.378761
ATCAACG46950.034.4488373
TCAACGC47100.034.4000324
CAACGCA47400.034.1705445
AACGCAG48950.033.1584976
TGGTATC6650.031.4561522
ACGCAGA52150.031.1238387
AGAGTAC53000.030.94772711
CGCAGAG52850.030.7116038
GTCGCTA450.00470574830.55371331
CAGAGTA54350.030.36803210
GCAGAGT54050.030.2198169
ACGGGAT2600.027.67096916
AGTACGG31100.026.86757513
GAGTACT30600.026.52137612
GAGTACG31000.026.51054612
AGTACTT31200.025.6838613
Produced by FastQC (version 0.11.5)