FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742695
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGC82190.47162584387973794No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC59540.34165473591190654No Hit
GCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGGGTGTTGTCGTAGGA55520.31858701608715234No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG51350.2946585604480417No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTG50660.29069917570200177No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47410.2720498997242776No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40970.23509564209457193No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC40940.23492349493170062No Hit
GGTTATGATGTACATTGGTACCAGCAACTTCCAGGAGCAGCCCCCAAGCTCCTCATCTATGGGAACGCCAATCGC36890.21168362794407514No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36820.21128195123070875No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCAC36550.20973262676486706No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35850.2057158596312034No Hit
GACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACTACTCAGG35850.2057158596312034No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT34890.20020715041932177No Hit
ACTCAGGGTGTTGTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAG33920.19464105881981644No Hit
GGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGT33830.19412461733120254No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTC33190.19045214452328146No Hit
GTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC33140.19016523258516263No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGGCGCCAACATCGGGGCAGGTTATGATGTACATTGGTACCAG32460.1862632302267465No Hit
TCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG32260.18511558247427118No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31980.1835088756208057No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG31730.18207431593021153No Hit
GAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACT31280.17949210848714203No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA30710.17622131239258734No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCAC30370.1742703112133793No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA29440.16893374916436898No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT27620.15849015461684346No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27580.1582606250663484No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG27100.1555062704604076No Hit
GTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGG26390.15143212093912015No Hit
TCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTAC26380.15137473855149639No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT25760.14781703051882286No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25180.1444888520366444No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGG24760.1420787917564462No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGG24340.139668731476248No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC24310.1394965843133767No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24120.13840631894852512No Hit
CCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG23830.13674222970743588No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT23630.13559458195496055No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23400.13427478703961393No Hit
GATTATTACTGCCAGTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGG22880.13129090288317807No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22480.1289956073782274No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22410.12859393066486105No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC22050.12652816471040546No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21730.1246919283064449No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA21680.12440501636832607No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC21450.12308522145297943No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTA21420.12291307429010813No Hit
ATCTATGGGAACGCCAATCGCCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCC21260.12199495608812785No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC21040.120732543560405No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTACGACAACACCCTGAGTAGTGTGGTGTTCGGCGGAGGGACCAAG20860.1196996605831772No Hit
CTCACTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCA20510.11769127701634537No Hit
GTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC20340.11671577642674136No Hit
GTCGTAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG20310.11654362926387003No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC20190.11585504061238484No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT20050.1150516871856521No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT20020.11487954002278081No Hit
CCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG19900.1141909513712956No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGC19490.11183827347872118No Hit
GTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCGG19430.11149397915297858No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19410.11137921437773106No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19400.11132183199010727No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19380.11120706721485973No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG19340.11097753766436468No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT19240.11040371378812702No Hit
GTATCAACGCAGAGTACGGGAGGCTCTGCTTCAGCTGTGGGCACAAGAGG18810.10793627112030504No Hit
GTAGTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCC18780.10776412395743375No Hit
GTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCTTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC18760.10764935918218621No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC18700.10730506485644362No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT18390.10552621084010684No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTC18340.10523929890198802No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAAGACGGTCAGCTTGGTCCCTCCGCCGAACACCACA18320.10512453412674048No Hit
CAGTGTCTGTGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGC18180.10432118070000775No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17980.10317353294753241No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17720.10168159086931447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9000.050.900391
TGGTATC9300.048.4744872
GGTATCA26800.042.2837141
GTATCAA67600.039.9467431
TATCAAC74350.036.195172
ATCAACG81100.033.2917483
TCAACGC81700.032.9394044
AACGCAG85150.031.6728046
CAACGCA88750.030.4293795
CGCAGAG90250.029.8829868
AGAGTAC89800.029.64929811
CAGAGTA90550.029.44250510
ACGCAGA93450.028.9332337
GCAGAGT96250.027.948769
ATAGCGG1403.8016879E-1027.59409763
AATAGCG1305.2823452E-926.98591462
ACGAACC1305.7079887E-926.7896852
GAGTACG67700.025.98186712
AGTACGG67600.025.9724613
CAAACGA1401.2809323E-824.83135249
Produced by FastQC (version 0.11.5)