FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences836483
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT28810.3444182368320695No Hit
GTAATAAACAGCCGTGTCTGCGGCGGTGACAGAACTCAGTCTCAGGGAGA25420.3038914120191325No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG24900.297674907918033No Hit
GTATGATAGTCTTCCTCCCACCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCAT23040.27543895094102333No Hit
CTATCATACTGTTGACAGTAATATGTCGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTC21600.25822401650720933No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19520.23335800010281144No Hit
CACCTGTACTGTCTCTGGTGGCTCCATCAGCAGAGACAATTACTACTGGG19350.23132568145437502No Hit
AGTCAGGACATTACCAATTATTTAAATTGGTATCAGCAGAAACCAGGGAA17410.20813333923104235No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17310.2069378576731386No Hit
GTCGGAGACAGAGTCACCATCACTTGTCAGGCAAGTCAGGACATTACCAATTATTTAAATTGGTATCAGCAGAAA16180.19342891606882626No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT15700.1876906045908883No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15590.18637557487719414No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15550.18589738225403266No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT15500.1852996414750808No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14810.17705081872554493No Hit
GTATCAACGCAGAGTACGGGATGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTT14580.17430121114236632No Hit
GTCTCTGGTGGCTCCATCAGCAGAGACAATTACTACTGGGGCTGGGTCCG13970.16700877363915345No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13930.16653058101599197No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13930.16653058101599197No Hit
GTTTATTACTGTGCGAGACACTTGTCCGGGCCGCTCACTTTGGTCCGGGGAGTTATCCCGTCTTCCTTTGACTA13480.16115091400542508No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13230.15816221011066572No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCTCCGCCGAAGGTG13220.15804266195487535No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC13210.15792311379908497No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGTCAGGCAAGTCAGG13210.15792311379908497No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12820.15326073572326035No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC12540.14991338736112986No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT12530.1497938392053395No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT12380.14800061686848387No Hit
ATCTCATACTGGGAACACCTACTACAAGTCGTCCCTCAAGAGTCGAGTCA12050.14405552772740152No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT11880.14202320907896515No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11690.13975179411894803No Hit
GTTTACAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTACTTTCACCATCAGCAGC11370.13592625313365606No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11340.13556760866628492No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10920.13054658612308917No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCCCAACCAGGACACAGCATGGAC10560.12624285251463568No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10510.1256451117356838No Hit
ATATTACTGTCAACAGTATGATAGTCTTCCTCCCACCTTCGGCGGAGGGA10500.12552556357989345No Hit
GTACTGTCTCTGGTGGCTCCATCAGCAGAGACAATTACTACTGGGGCTGGGTCCGCCAGTCCCCAGGGAAGGGG10310.12325414861987631No Hit
GTAATATGTCGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA10310.12325414861987631No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10260.12265640784092445No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA10220.12217821521776295No Hit
ATCATACTGTTGACAGTAATATGTCGCAATATCTTCAGGCTGCAGGCTGC10160.1214609262830207No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9940.11883086685563246No Hit
GTATCAACGCAGAGTACGGGGCTTTCTGAGAGTCATGGATCTCATGTGCAAGAAAATGAAGCACCTGTGGTTCTT9910.11847222238826133No Hit
GGGTTCCCTGGCCCCAGTAGTCAAAGGAAGACGGGATAACTCCCCGGACCAAAGTGAGCGGCCCGGACAAGTGT9660.11548351849350197No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC9600.11476622955875972No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC9510.11369029615664633No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9220.11022339963872547No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9070.10843017730186985No Hit
ACATATTACTGTCAACAGTATGATAGTCTTCCTCCCACCTTCGGCGGAGGGACCAAGGTGGAGATCAGACGAA9010.1077128883671276No Hit
CAGTAATATGTCGCAATATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTCCCAGATCCACTTCCA8930.1067565031208046No Hit
GGGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTT8890.10627831049764311No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA8880.10615876234185274No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8870.10603921418606235No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8690.10388734738183561No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC8660.1035287029144645No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATC8650.10340915475867411No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA8620.10305051029130301No Hit
GGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCT8570.10245276951235112No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8550.10221367320077036No Hit
GTCCTGTCCCAGGTGAAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCC8470.10125728795444737No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTA8450.10101819164286663No Hit
GATCTACGATGCATCCAGTTTACAAACAGGGGTCCCATCAAGGTTCAGTGGAAGTGGATCTGGGACAGATTTTA8380.10018135455233398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)