FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences924687
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24380.26365678332235665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.13647861384446847No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10180.11009130657184539No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9430.10198045392657192No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCGC200.007227614551.98554242
ATCGCGT605.6061253E-946.0035537
TCGAACG306.4498023E-445.79707711
TATCGCG651.1426891E-842.4417636
GTATCAA47450.041.5638351
GTGGTAT5900.041.3472181
GGTATCA22750.036.5488661
TATCAAC53950.036.3650862
ACCGATC2650.035.04325527
ATCAACG56600.034.783893
TCAACGC56850.034.6309284
CAACGCA58500.033.536695
AACGCAG59100.033.2543536
CGATCAG2850.031.38915629
CAGTTCG4400.030.41645470
CGCAGAG64400.030.4010098
ACGCAGA65300.029.9820087
AGAGTAC66150.029.80444711
CGCGTAT703.8154754E-529.61070339
TCGCGTA703.8367674E-529.58656538
Produced by FastQC (version 0.11.5)