FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990676
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51280.5176263480693991No Hit
GATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA45180.4560522310018613No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC35790.3612684671880615No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33080.3339134086219915No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32580.32886634984596375No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31930.3223051734371278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30810.31099976177882577No Hit
AGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC30430.30716399710904474No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC30260.3054479971251953No Hit
GTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT29230.29505105604657833No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29200.29474823252001664No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG27240.2749637621179881No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26750.270017644517481No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG26090.2633555269331245No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA26000.26244705635343946No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25620.25861129168365843No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA24210.24437858593526038No Hit
CTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC22460.2267138802191635No Hit
CTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT22450.22661293904364294No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22260.22469505670875242No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21670.21873952735303975No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT21290.2149037626832587No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTA21240.21439905680565594No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT19890.20077199811038118No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19790.19976258635517563No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19200.193807056999463No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC18800.18976940997864084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18770.18946658645207917No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18260.18431858650053096No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG18140.1831072923942843No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18030.18199693946355822No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17010.17170093956046176No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16240.1639284690453791No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16110.16261623376361192No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16110.16261623376361192No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15910.16059741025320085No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAG15830.15978988084903642No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15570.15716541028550204No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG15110.15252211621155656No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15030.15171458680739214No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC14890.15030141035010436No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14710.1484844691907344No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14520.1465665868558439No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCAT14410.14545623392511778No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14340.14474964569647392No Hit
GCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAG14160.14293270453710397No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC14050.14182235160637788No Hit
ACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA14030.14162046925533675No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14030.14162046925533675No Hit
GTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGG13250.13374705756473357No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGG12980.13102164582567863No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12920.1304159987725553No Hit
CACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAAC12620.1273877635069387No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12450.12567176352308929No Hit
CACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC12360.1247632929434043No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12090.12203788120434934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12020.12133129297570548No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11680.11789929300800665No Hit
GCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGA11530.11638517537519834No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11370.1147701165668695No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.11416446951374618No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC11310.11416446951374618No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11290.11396258716270508No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11210.11315505775854064No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11170.11275129305645841No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11000.11103529307260901No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC10980.1108334107215679No Hit
ATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA10980.1108334107215679No Hit
GTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG10980.1108334107215679No Hit
ACCTTGATGGGACCCCAGTCTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTG10920.11022776366844458No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10830.1093192930887596No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT10800.10901646956219793No Hit
TCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT10680.1078051754559513No Hit
CCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCA10440.105382587243458No Hit
GGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCC10290.10386846961064969No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC10260.10356564608408805No Hit
CAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT10210.10306094020648526No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10130.10225341080232082No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC9970.10063835199399199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGG300.002878089765.59437670
GTATCAA29700.042.2394141
GTGGTAT4400.040.6192741
CGTAGCG350.001307106339.67476347
TATCAAC33550.037.392272
TCGTATG1500.036.8905740
TATGCCG1750.035.634643
ATCAACG35600.035.432153
TCAACGC35950.035.0871964
CGTATGC1800.034.6060141
CAACGCA36500.034.5584835
AACGCAG37400.033.8187646
TCCGAGC2450.033.66022513
CTCGTAT1850.033.63822639
GCCTATC1950.033.57701533
TGCCGTC1900.032.85143345
GCCGTCT1800.032.7650646
TATCTCG1800.032.58572836
ATCTCGT1850.031.72754137
CGCAGAG39900.031.6917928
Produced by FastQC (version 0.11.5)