FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences990676
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48140.4859308189559452No Hit
GATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCA40000.40376470208221454No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31590.31887317346942895No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29940.30221787950853757No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29320.2959595266262633No Hit
AGCTTAGAGACTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGC28580.2884898796377423No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG28300.2856635267231668No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAATTG27780.280414585596098No Hit
GTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT27510.27768917385704306No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27070.2732477621341387No Hit
CTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC26580.2683016445336316No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26130.26375929163520667No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24690.24922376236024696No Hit
CTTTCATACTGTTGGCAGTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC23500.23721176247330106No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23220.23438540955872553No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21600.21803293912439586No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20660.20854446862546383No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20460.20652564511505275No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTA20450.20642470393953222No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCG20340.2053143510088061No Hit
CTCTAAGCTCGACGCCCTATAGATCAGGAGCTTAGGGGCTTTCCCTGGTT20280.20470870395568277No Hit
GTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGA20120.20309364514735392No Hit
GTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA19960.20147858633902507No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19620.19804658637132627No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC19110.19289858641977803No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18120.1829054100432432No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17980.18149223358595545No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17830.17997811595314714No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16940.17099435133181787No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16830.16988399840109178No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG16740.1689755278214068No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16710.16867270429484513No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16500.16655293960891351No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16220.163726586694338No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC16080.16231341023705026No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15850.15999176320007752No Hit
ACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA15600.15746823381206368No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC15160.15302682208915933No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15010.151512704456351No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14860.1499985868235427No Hit
GCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGACCAAG14490.14626376332928223No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14040.14172141043085731No Hit
GTAATAAGTTGCCATGTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG13830.13960164574492567No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA13760.1388950575162818No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT13750.13879411634076125No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13740.1386931751652407No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13630.13758282223451462No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13070.13193011640536362No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG12900.1302141164215142No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12850.12970941054391144No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAG12830.1295075281928703No Hit
CACCATCAGCAGCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAAC12820.12940658701734978No Hit
ATTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12490.12607552822517148No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA12460.12577270469860982No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT12170.12284541060851378No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGGCGTCGAGCTTAGAGACTGGGGTCCCAT11740.11850494006112999No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC11730.11840399888560943No Hit
GTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTATAGGG11380.11487105774239004No Hit
GGCCAAAATTGTACGAGTAACTTTCATACTGTTGGCAGTAATAAGTTGCC11320.11426541068926671No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11210.11315505775854064No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11170.11275129305645841No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA11170.11275129305645841No Hit
GCCTGCAGCCTGATGACATGGCAACTTATTACTGCCAACAGTATGAAAGTTACTCGTACAATTTTGGCCAGGGGA11040.11143905777469122No Hit
GTCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC10870.1097230577908418No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10700.1080070578069924No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10600.10699764605178687No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10590.1068967048762663No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGACATGG10580.10679576370074574No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC10560.10659388134970464No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAGT10400.10497882254137578No Hit
CACTATCACTTGCCGGGCCAGTCAGAGGATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC9970.10063835199399199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)