FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2396613
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39830.16619287302539043No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36070.15050406552914466No Hit
GTAATAGACCGCCGTGTCATCGGCTCTCAGTCTGTTCATTTGCAGATACA28200.11766605622184308No Hit
GGTGTATACTACGGAGCCTCTGTGAAGGGCCGTTTCACCAGCTCCAGGGACAACGCCAAGAACTCAGTGTATCTG27190.11345177548481962No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATG27130.1132014221737093No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27050.11286761775889557No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26790.11178275341075092No Hit
GTCTATTACTGTGTGAGAGATGGCGCCTTCGGATATAGTGGCTACGATCGTGAAGAGTACTGGGGCCAGGGAACC25520.10648360832558282No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25300.10556564618484501No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25220.1052318417700313No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25130.10485631180336584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35050.040.6473271
GTATCAA82550.038.894151
TATCAAC93150.034.5240252
TCAACGC98400.032.636334
ATCAACG98500.032.6263543
CAACGCA101700.031.564165
TGGTATC17450.031.3396682
AACGCAG103250.031.3211176
GTGGTAT17800.031.3194921
CGCAGAG110750.029.1070528
ACGCAGA112300.028.6747387
GCAGAGT115350.027.886789
CAGAGTA117900.027.34186610
AGAGTAC124750.025.61877311
AGTACGG80150.025.400713
GAGTACG81850.024.99740412
TACGGGA35450.022.08670415
GTACGGG92500.021.82879814
AGTACTT52550.021.82067913
TTCGGGG16200.021.81040870
Produced by FastQC (version 0.11.5)