FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683043
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35310.5169513485973797No Hit
ACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTC29040.4251562493137328No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24290.35561450743218215No Hit
GAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23650.3462446727365627No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23270.3406813333860387No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23250.34038852605180053No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23220.3399493150504434No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG21690.31754955398122814No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG19660.2878296095560602No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19330.28299828854113135No Hit
CCTCAAGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAG18470.27040757316889275No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18080.26469783015124965No Hit
GTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT16850.24669017909560598No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCG16410.24024841774236763No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCCTGGCCGAACGTC16210.23732034439998656No Hit
ATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAA15710.23000016104403384No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG15660.22926814270843857No Hit
GTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATCCA14950.21887348234298573No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13440.19676652860800858No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13310.19486328093546088No Hit
GTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGTAC13260.1941312625998656No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT13240.19383845526562749No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12770.18695748291103195No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12670.18549344623984143No Hit
ATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACT12600.18446862057000804No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC12530.18344379490017465No Hit
AGGTAGTGTAACTCTGTTGACAGTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGT12200.1786124738852459No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11890.1740739602045552No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.1724635198662456No Hit
CCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAG11690.17114588686217413No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11660.17070667586081698No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11620.17012106119234074No Hit
ACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGAA11450.1676321988513168No Hit
ACACTACCTTGAGGACGTTCGGCCAGGGGACCAAGGTGGAAATCAAACGA10950.1603120154953641No Hit
GACTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTAC10870.1591407861584117No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC10730.15709113481874495No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10700.15665192381738777No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCA10580.15489507981195913No Hit
GTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCACTGAACCTTGATGGGAC10570.15474867614484006No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10440.15284542847229238No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCACTGTA10240.1499173551299113No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAAGCG10230.14977095146279223No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10220.1496245477956732No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA10150.1485997221258398No Hit
GTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTTCGGCCAGGGGA10100.14786770379024453No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10070.14742849278888737No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC9880.14464682311362537No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA9840.14406120844514914No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9790.14332919010955386No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG9450.13835146542750604No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9430.13805865809326792No Hit
GTAATAAGTTGCAAAGTCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAGTCTGTCTCAGATCCACTGCCA9190.13454497008241062No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9180.13439856641529158No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC9100.13322733707833914No Hit
CACCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAGAGTTACACTACCTTGAGGACGTT9070.13278812607698198No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT9050.13249531874274387No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8880.13000645640171996No Hit
GAGTATTAGTACCTTTTTAAATTGGTATCAGCAGAAACCAGGGAGAGCCCCTAAGCTCCTGATCTTTGCTGCATC8820.1291280343990056No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACC8670.1269319793922198No Hit
CTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGTATTAGT8640.12649276839086265No Hit
GTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTC8570.1254679427210293No Hit
GTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA8320.12180785104305293No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8300.12151504370881482No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC8220.12034381437186238No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACT8100.11858697036643373No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8070.11814775936507657No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8050.11785495203083848No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGC8020.1174157410294813No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC8020.1174157410294813No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8000.11712293369524321No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG7840.11478047502133833No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAAGCGGGTAACTCCCAGGAGAG7800.11419486035286212No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT7760.11360924568438589No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT7760.11360924568438589No Hit
CTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTC7750.11346284201726685No Hit
GAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGAGACAGACTTCACTCTCACCATCAGCAGTCTGCAAC7740.11331643835014779No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCA7680.11243801634743347No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC7440.10892432833657618No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA7390.10819231000098091No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT7230.10584985132707604No Hit
CCATCAGCAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAACAG7210.10555704399283794No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC7120.10423941098876643No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC7100.10394660365452835No Hit
CCATCCTCCCTGTCTGCATCTATAGGAGACAGAGTCACCATCACTTGCCG7020.10277537431757591No Hit
GTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCA7020.10277537431757591No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6970.10204335598198065No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGCTTGGAGGGCGTTATCCACCTTCCA6930.10145774131350442No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC6850.10028651197655199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAA200.00696081852.48083556
ATCGCTC200.00737493751.71945634
GTATCAA23250.043.0719071
GTGGTAT3000.041.154321
CGTATGC702.0976586E-839.61535341
TCGTATG557.212453E-637.76991340
TATCAAC26800.037.3664862
ATCAACG28550.034.8358273
GCCGAAG400.00250495734.7509144
TGTCGTA400.002575030634.5559838
TCAACGC28800.034.533434
CTTATAC1201.8189894E-1234.2952651
TCTGTCG400.00268015634.2751858
CAACGCA29800.033.374595
CGAGACA1253.6379788E-1232.93310523
ACGAGAC1253.6379788E-1232.9282822
AACGCAG30250.032.7647326
TCCGAGC1208.367351E-1131.41891713
TTGCCGA450.004314891531.09975256
TATGCCG901.8838728E-730.86449843
Produced by FastQC (version 0.11.5)