FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140825
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12860.11272543992286282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47050.037.7289921
GTGGTAT7600.036.212751
GGTATCA21200.034.8889351
TATCAAC54350.032.5362662
TCAACGC56450.031.3258824
ATCAACG57350.030.8942743
CAACGCA58300.030.5088715
TGGTATC8650.030.227462
AACGCAG60100.029.8241046
ACGCAGA65050.027.5497997
CGCAGAG66200.027.0192558
AGAGTAC67800.026.58340311
CAGAGTA68600.026.27339210
GCAGAGT69250.025.9274439
GACGGAC2050.025.17779526
TCGTATG1551.4533725E-924.58576640
TATCTCG1752.382876E-1023.68658436
GAGTACG44000.023.45187412
AGTACGG44050.023.34716813
CGAGACG2250.022.93373923
Produced by FastQC (version 0.11.5)