FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140825
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17420.15269651348804594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT8650.044.4972761
GGTATCA24900.039.3348051
GTATCAA56400.038.022181
TGGTATC10200.036.0318262
TATCAAC64500.032.963522
TCAACGC66500.031.8868964
ATCAACG66900.031.665783
CAACGCA67800.031.3643325
AACGCAG68600.031.197196
ACGCAGA74500.028.7725777
CGCAGAG75650.028.1991798
AGAGTAC77500.027.79034211
CAGAGTA78300.027.5076110
GCAGAGT78750.027.219779
GAGTACG50150.023.3889212
AGTACGG50250.023.20688413
GAGTACT42200.023.08133112
AGTACTT43700.021.97609913
TACTCGC800.002999689221.4365085
GTACTTT45350.021.25402314
Produced by FastQC (version 0.11.5)