FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919193
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10430.11346909734952289No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5250.043.2198521
GGTATCA15900.040.0012251
GTATCAA37400.039.7111
TGGTATC6250.037.404822
TATCAAC45200.032.782152
TCAACGC46750.031.621724
ATCAACG47300.031.326713
ACGCTAA1202.8420784E-530.91221270
CAACGCA48400.030.7568045
AACGCAG49300.030.1255866
ACGCAGA53100.028.0237757
CGCAGAG54400.027.668458
AGAGTAC55600.027.19490211
CAGAGTA57050.026.56394210
GCAGAGT57600.026.1313139
GAGTACG36100.024.6548412
AGTACGG36250.024.45802113
CGTAAAC2057.4578566E-1121.84796132
GAGTACT28350.021.81870812
GTACGGG40350.021.5469914
Produced by FastQC (version 0.11.5)