FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences919193
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18360.19974042448103937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6500.043.369351
GGTATCA18400.041.6648141
GTATCAA42250.038.4872441
TGGTATC7150.038.4422
TATCAAC48200.033.787292
ATCAACG50450.032.2017973
TCAACGC50500.032.0826844
CAACGCA51750.031.2975165
AACGCAG53100.030.7569586
ACGCAGA56550.028.9412147
CGCAGAG57900.028.4441938
AGAGTAC59450.027.70258911
CAGAGTA60700.027.41473210
GCAGAGT61750.026.6151879
GTACGGA658.9353434E-426.39436714
AGTACGG37550.025.31050513
GAGTACG37300.025.29479612
TACGGGC10250.025.10956815
ACGGGCT5550.023.49592216
GTACGGG42400.021.85005414
Produced by FastQC (version 0.11.5)