FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247539
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT49950.4003882844544339No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33890.27165483403725255No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33760.27061278244607984No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC31280.2507336443990929No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT31160.24977175062262583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30830.2471265427373413No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30470.24424086140793994No Hit
GTAATATACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGCCTCAGGGAGA28050.22484267024918658No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC27950.2240410921021307No Hit
GTATATTACTGTGCGAGACTTATTGATACTACGGTGGGTCCGTTTGACTA27930.2238807764727195No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27620.2213958842168461No Hit
CCCCTAAGTTCCTGATCTATAAGGCGTCTAGCTTAGAAAGTGGGGTCCCA27410.2197125701080287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26810.21490310122569317No Hit
GTCTCTGGTGGCTCCGTCAGCGCTGTCTCTCCTGACTCCGTCTTCAGCAATACTTTCTACTGGGGCTGGGTCCGC24320.19494380536400063No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGAGTATTAGTAACTGGCTGGCCTGGTATCAGCAGAAA23940.19189780840518814No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23130.18540502541403514No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGAGTATTAGTAACTGG22790.182679659714045No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGA22660.18163760812287233No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC22540.1806757143464052No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG21160.16961393591703344No Hit
CACCATCACCAGCCTGCAGCCTGAAGATTCTGCAACTTACTTCTGCCAACAGGTTCAAAGTTATCCGGGGACGTT20920.16769014836409923No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20530.16456399359058113No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC20220.16207910133470776No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19970.16007515596706798No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19760.1583918418582505No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG19510.15638789649061072No Hit
GAATTCACTCTCACCATCACCAGCCTGCAGCCTGAAGATTCTGCAACTTACTTCTGCCAACAGGTTCAAAGTTAT19340.15502521364061564No Hit
GAGTATTAGTAACTGGCTGGCCTGGTATCAGCAGAAACCAGGGCACGCCCCTAAGTTCCTGATCTATAAGGCGT19260.1543839511229709No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19120.15326174171709261No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19110.15318158390238704No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC18930.15173874323768635No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18910.15157842760827517No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18600.1490935353524018No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.14580706494947251No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17890.14340233050830475No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT17880.14332217269359915No Hit
GTATCAACGCAGAGTACGGGAGGCTTTCTGAGAGTCATGGATCTCATGTGGAAGAAAATGAAGCACCTGTGGTT17750.14228012110242644No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17700.1418793320288985No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17650.14147854295537055No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17550.14067696480831463No Hit
CACCTGCACTGTCTCTGGTGGCTCCGTCAGCGCTGTCTCTCCTGACTCCGTCTTCAGCAATACTTTCTACTGGGG17120.13723017877597413No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16830.13490560214951197No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16820.13482544433480637No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC16570.13282149896716658No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16540.13258102552304976No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16480.13210007863481624No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC16130.1292945551201205No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT15860.1271302941230695No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG15650.12544698001425206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15560.12472555968190173No Hit
ACTTAGGGGCGTGCCCTGGTTTCTGCTGATACCAGGCCAGCCAGTTACTA15380.12328271901720106No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15110.12111845802015007No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT14830.11887403920839347No Hit
TCTATAAGGCGTCTAGCTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14480.11606851569369776No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14220.11398441251135236No Hit
AGCTTAGAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACCATCACCAGC14070.11278204529076848No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14000.1122209405878293No Hit
ATCCGGGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13800.11061778429371748No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13780.11045746866430628No Hit
GTATTAGTAACTGGCTGGCCTGGTATCAGCAGAAACCAGGGCACGCCCCT13620.1091749436290168No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTGAGAGTATTAGTAA13570.10877415455548885No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGGGCTTGCCGGCCGTCGCACTCATTTA13570.10877415455548885No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG13520.10837336548196089No Hit
TCACCAGCCTGCAGCCTGAAGATTCTGCAACTTACTTCTGCCAACAGGTT13460.10789241859372734No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13390.10733131389078819No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13230.1060487888554987No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13150.10540752633785397No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13110.1050868950790316No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC13080.10484642163491482No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG13000.10420515911727009No Hit
GCAGAAACCAGGGCACGCCCCTAAGTTCCTGATCTATAAGGCGTCTAGCTTAGAAAGTGGGGTCCCATCAAGGTT12870.10316310752609738No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG12790.10252184500845263No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC12740.10212105593492468No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12680.10164010904669112No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12620.10115916215845758No Hit
TTCTAAGCTAGACGCCTTATAGATCAGGAACTTAGGGGCGTGCCCTGGTT12520.10035758401140164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4000.050.708691
CTTATAC2300.043.347151
AACGGAG10650.038.83061270
TATCAAC45150.036.0920032
TATACAC2800.035.6065863
CTAGCGC2750.035.0305328
ACGAGAC2850.034.9805122
CGCGTAT400.002579367534.54637539
GTATCAA47750.034.486771
TAGCGCT2800.034.4174129
ATCAACG48650.033.919453
ACTAGCG2950.033.81370527
TCAACGC49050.033.6428384
CAACGCA49700.033.2720155
TTATACA3100.033.269782
ACGGGAA1350.033.096216
AACGCAG50850.032.5195476
AGACTAG3100.032.16465825
CACGAGA3000.032.08428621
GCGTATG652.2915932E-531.9069640
Produced by FastQC (version 0.11.5)