FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1837068
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT30180.16428352135032565No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAGCATCCTCAGCCTCCACCC29170.15878563014542738No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24860.13532433203343588No Hit
CCCTTGGACAGGCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA23190.126233759447119No Hit
GTACACACTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA20940.1139859820104645No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18800.10233698480404646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT11650.048.9725761
TGGTATC12350.046.169082
GTATCAA83800.041.9978141
GGTATCA32200.038.425311
TATCAAC93200.037.4076352
ATCAACG100600.034.730943
TCAACGC101500.034.4314584
CAACGCA103200.033.9833265
AACGCAG105350.033.317796
ACGCAGA112450.031.244627
CGCAGAG112700.031.1448928
AGAGTAC113800.030.93420611
CAGAGTA114650.030.67496910
GCAGAGT115950.030.2127979
GTACGGT756.4263455E-527.4325614
AGTACGG82050.027.19873213
GAGTACG83350.027.01981712
GAGTACT51550.024.60343212
AGTACTT52050.023.90738913
TACGGGG44900.023.82978215
Produced by FastQC (version 0.11.5)