FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015179
Sequences flagged as poor quality0
Sequence length20-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14240.14027082908531402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46050.041.772041
GGTATCA17750.040.4459651
TATCAAC54150.035.5235862
GTGGTAT6750.035.1133731
TCAACGC56050.034.3806884
ATCAACG56250.034.319513
TGGTATC7150.033.6294142
CAACGCA57800.033.4586075
AACGCAG59800.032.454476
CTCGCTA652.3889968E-531.71241623
ACGCAGA62900.030.9034847
CGCAGAG63900.030.3123728
AGAGTAC65650.029.55666511
CAGAGTA66000.029.45195810
GCAGAGT66900.029.0044089
ACGAGTA900.0085458427.03938970
GAGTACG40650.026.10595712
AGTACGG41150.025.78875213
GAGTACT35600.024.69628712
AGTACTT36650.023.89504613
Produced by FastQC (version 0.11.5)