FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061973
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12870.12118952176750257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44200.040.0798151
GGTATCA19300.039.2108421
GTGGTAT6200.038.2823141
TATCAAC50350.035.184272
TCAACGC53050.033.5880784
ATCAACG53300.033.4305343
CAACGCA54400.032.8177835
AACGCAG56500.031.9024186
TGGTATC7650.030.126882
ACGCAGA60350.029.9171757
CGCAGAG61300.029.2277478
AGAGTAC63650.028.20266711
CAGAGTA64700.027.85127610
GCAGAGT65000.027.6698259
TATACAC3600.025.798953
AGTACGG38600.025.03439513
GAGTACG39050.024.8339712
CTTATAC3550.024.224371
GAGTACT35800.023.1500612
AGTACTT36050.022.89412713
Produced by FastQC (version 0.11.5)