FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061973
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26600.2504771778566875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53250.041.1729621
GTGGTAT7500.039.8629151
GGTATCA22400.038.813571
TATCAAC61000.035.689682
ATCAACG63250.034.2872473
TCAACGC63500.034.1772964
CAACGCA64650.033.672245
AACGCAG66200.033.1412126
TGGTATC9050.032.63122
ACGCAGA70600.031.0757527
CGCAGAG72650.030.151698
AGAGTAC73700.029.81514411
CAGAGTA74200.029.66043710
GCAGAGT74550.029.567179
TCGGTAC500.00794096927.4514983
GAGTACT41300.026.47811512
ACACCGT658.985434E-426.3695686
AGTACTT41950.025.98857313
AGTACGG44050.024.90527713
GAGTACG44500.024.65110212
Produced by FastQC (version 0.11.5)