FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012256
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.20824771599279235No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16280.1608288812316252No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT14800.146208073846932No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12630.12477080896532103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.12022650396737586No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG11990.11844829766383207No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11170.11034758005879935No Hit
GATCTATGCTGCATCTATTTTGGAAAGTGGGGTCCCATCAAGATTCAGCGGCAGTGGATCTGGGACAGAATTCA10890.10758148136439795No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT10780.10649479973445451No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10220.10096260234565169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40100.041.456381
GTGGTAT4950.039.5512351
TATCAAC45750.036.186482
ATCAACG48750.034.1709863
TCAACGC48950.033.96124
CGCTAAT3300.033.39208232
CAACGCA50000.033.316715
CGCTCAT952.6441127E-432.49475570
AACGCAG51550.032.314956
GGTATCA20950.031.9698751
AAATCGC450.003896535131.75602566
CTTATAC4150.031.4503781
TATACAC4600.031.3604183
AATCGAC450.004650200730.62913134
GGTCCGT450.0047205230.53530319
CGCAGAG56000.029.8653058
ATAGCGG604.7077247E-429.4519563
TCTCCGA4100.029.3163711
TCGTATG3200.029.1996540
AGAGTAC58300.028.68708611
Produced by FastQC (version 0.11.5)