FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439918
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT53120.3689098962579814No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44360.308073098607004No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT40340.2801548421507336No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC36750.255222866857696No Hit
AGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT35520.24668071376286707No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC34710.24105539343212598No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCGATCATTTAAATTGGTATCAGCAGAAA34490.23952752865093707No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG34220.23765242187402338No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32200.22362384524674322No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG30910.2146650017570445No Hit
CTACTGGAGTTGGGTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCCATTACGGTGGGAGCAC30160.20945637182117316No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29960.20806740383827413No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT29100.2020948415118083No Hit
ACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGA28900.2007058735289093No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG28800.20001138953745978No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT28620.19876131835285066No Hit
GTGCAGCCACAGTTCGCGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG27620.19181647843835553No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27610.19174703003921056No Hit
GTTACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACG26010.18063528617601837No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGATC25990.18049638937772844No Hit
GTCTCTGGTGCCTCCACCAACAGTGGTGGTCACTACTGGAGTTGGGTCCG25950.18021859578114865No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25900.1798713537854239No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT25700.17848238580252485No Hit
GAGTTACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGAACTGTGGCTGCACCAT25360.17612114023159653No Hit
ACCTTGAAGGGACCCCACTTTGTAAACTGGATGCAGCAAAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG24420.1695929907119711No Hit
GGCCAAAAGTGCACAAGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCA24300.16875960992223168No Hit
CTTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTG23740.1648704995701144No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23670.16438436077609975No Hit
ACTCTAAGTCGTCCCTCAAGAGCCGAGTTACCATGTCAGCAGACACGTCT23190.1610508376171421No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22810.15841179844963393No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22760.15806455645390918No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACAAGGTA22320.1550088268915313No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC22260.15459213649666162No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGAA22190.15410599770264696No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22050.15313372011461762No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21880.15195309732915346No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21340.1482028837753261No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA21230.14743895138473162No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC21170.14702226098986193No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT21060.14625832859926746No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21030.14604998340183262No Hit
GTCCTGATCTTTGCTGCATCCAGTTTACAAAGTGGGGTCCCTTCAAGGTT21030.14604998340183262No Hit
CACCTGCACTGTCTCTGGTGCCTCCACCAACAGTGGTGGTCACTACTGGA20980.14570274140610784No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC20530.14257756344458505No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT19860.1379245207018733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19740.1370911399121339No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19590.13604941392495962No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT19320.13417430714804593No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19290.13396596195061106No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGAACTGTGGCTGCACCA19260.1337576167531762No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19100.132646442366857No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATGATCGCTA18870.13104912918652312No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA18670.1296601612036241No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA18390.12771560602756546No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG18100.12570160245236187No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18050.12535436045663712No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACAGTAC17930.12452097966689771No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17830.12382649567544818No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC17790.1235487020788684No Hit
AGTCAGAGCATTAGCGATCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTTTG17550.12188194049938955No Hit
GAGCATTAGCGATCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTTTGCTGCATC17390.12077076611307033No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17390.12077076611307033No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT17380.12070131771392538No Hit
GCCACAGTTCGCGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACAA16920.11750669135325761No Hit
GTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA16390.11382592619857519No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16340.11347868420285044No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16300.11320089060627063No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16180.11236750981653122No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16110.11188137102251655No Hit
CCAATACACGGCCGTGTCCGCGGCAGTCACAGACCTCAGGTTCAGGGAGAACTGGTTCTTAGACGTGTCTGCTGA16100.11181192262337161No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16020.111256335430212No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15930.11063129983790744No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15920.11056185143876247No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15230.10576991189776085No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAAC15200.10556156670032599No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15170.10535322150289114No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC14980.10403370191913706No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC14930.1036864599234123No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14740.10236694033965822No Hit
GGGTTCCCTGGCCCCATGAGTCAAAGTATATGTAGCCATAGTCGACCTCTGAGGCTCTCGCACACCAATACACGG14730.10229749194051328No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC14610.10146411115077388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT5800.057.02461
GTATCAA44750.039.2641451
TATCAAC50250.034.9677932
TCAACGC53750.033.139524
ATCAACG53500.033.0367773
CAACGCA55150.032.298265
CTTATAC1500.032.1555371
AACGCAG55650.031.8842456
ACGAGAC1450.030.8872522
AATCTCG1251.2369128E-1030.3951536
CGTATGC1151.6480044E-930.09446541
GACAGGT3100.030.0068951
CGCAGAG60450.029.4013218
AGAGTAC60250.029.32639311
CTTGTAT953.2467142E-729.00949316
GCAGAGT61650.028.8838989
TCGTATG1202.6102498E-928.82745440
CACGAGA1700.028.37253421
ACGCAGA62700.028.2913117
CGTCAGA1850.027.9353185
Produced by FastQC (version 0.11.5)