FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1439918
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50830.3530062128537875No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG40920.28418284930114074No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT38880.2700153758755707No Hit
AGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT33350.2316104111484126No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT32360.22473501963306244No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCGATCATTTAAATTGGTATCAGCAGAAA30280.21028975261091257No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29610.20563670986820082No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28820.20015028633574966No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28220.1959833823870526No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC28040.19473331120244347No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT27830.1932748948203995No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27490.19091364924947116No Hit
GTGCAGCCACAGTTCGCGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG26680.1852883289187301No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT25940.1801491473820037No Hit
ACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGA25920.18001025058371378No Hit
CTACTGGAGTTGGGTCCGCCAGCACCCAGGGAAGGGCCTGGAGTGGATTGGGTACATCCATTACGGTGGGAGCAC25820.17931576659226428No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGATC24620.17098195869487012No Hit
GTCCTGATCTTTGCTGCATCCAGTTTACAAAGTGGGGTCCCTTCAAGGTT24440.169731887510261No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24390.16938464551453625No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24050.1670233999436079No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC23370.16230090880175121No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT23090.16035635362569256No Hit
GAGTTACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGAACTGTGGCTGCACCAT23010.15980076643253296No Hit
GTCTCTGGTGCCTCCACCAACAGTGGTGGTCACTACTGGAGTTGGGTCCG23000.15973131803338803No Hit
GTTACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACG22970.15952297283595315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22810.15841179844963393No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT22470.15605055287870562No Hit
GTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA22150.15382820410606715No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22130.15368930730777725No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21890.1520225457282984No Hit
ACTCTAAGTCGTCCCTCAAGAGCCGAGTTACCATGTCAGCAGACACGTCT21340.1482028837753261No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21040.14611943180097756No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT20850.14479991221722346No Hit
CTTCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTG20710.14382763462919415No Hit
GGCCAAAAGTGCACAAGGTACTGTAACTCTGTTGACAGTAGTAAGTTGCA20640.14334149583517952No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCTTGTGCACTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGAA20570.14285535704116484No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACAAGGTA20430.14188307945313552No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA20280.14084135346596127No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19880.1380634175001632No Hit
CACCTGCACTGTCTCTGGTGCCTCCACCAACAGTGGTGGTCACTACTGGA19420.13486879113949543No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCACGCGAACTGTGGCTGCACCA19330.1342437555471909No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC19090.13257699396771205No Hit
ACCTTGAAGGGACCCCACTTTGTAAACTGGATGCAGCAAAGATCAGGACCTTAGGGGCTTTCCCTGGTTTCTG18950.13160471637968274No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18950.13160471637968274No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC18870.13104912918652312No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18770.1303546451950736No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG18360.1275072608301306No Hit
CCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATGATCGCTA18000.12500711846091236No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17920.12445153126775274No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17790.1235487020788684No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT17640.12250697609169411No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17590.12215973409596935No Hit
GATCAGGACCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAAT17270.11993738532333093No Hit
GTTCAGTGGCAGTGGATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA17150.11910400453359149No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAACAGAGTTACAGTAC16930.11757613975240257No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16600.11528434258061918No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16320.11333978740456054No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16190.11243695821567616No Hit
CCAATACACGGCCGTGTCCGCGGCAGTCACAGACCTCAGGTTCAGGGAGAACTGGTTCTTAGACGTGTCTGCTGA16120.11195081942166152No Hit
CAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT16070.11160357742593675No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT15870.11021460944303774No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15770.10952012545158822No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCTCAGTCAGGACACAGCATGGAC15670.10882564146013869No Hit
AGTCAGAGCATTAGCGATCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTTTG15520.10778391547296444No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCC15500.10764501867467453No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG15410.10701998308236997No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15370.10674218948579016No Hit
GCCACAGTTCGCGTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTGCACAA15330.10646439588921036No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14860.10320032112939766No Hit
GAGCATTAGCGATCATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGGTCCTGATCTTTGCTGCATC14810.10285307913367289No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14730.10229749194051328No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14730.10229749194051328No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC14500.10070017876017939No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14480.1005612819618895No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14470.10049183356274453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4700.057.1669351
TGCGTTA200.00743000351.62518725
GTATCAA46950.043.5811841
TATCAAC53150.038.604172
ATCAACG56250.036.33153
TCAACGC56250.036.2489134
CAACGCA57950.035.3438845
AACGCAG58650.035.0380176
CGCAGAG62900.032.4520538
AGAGTAC63350.032.27577611
GCAGAGT64350.031.6140069
ACGCAGA66350.030.7646437
CAGAGTA67950.030.24252710
GCGTTGA1151.7334969E-929.953691
GAGTACG50900.027.81543412
AGTACGG50550.027.80794113
CGGGATA5500.027.5190817
GCCCGAT1000.00846597227.0924870
TCGCTAT1404.820322E-1027.03518524
TGAGCGG2805.802576E-1026.60868570
Produced by FastQC (version 0.11.5)