FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712217
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43500.2540565827812713No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG38850.2268988101391354No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35030.20458855390409042No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31800.18572412258492937No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31410.18344637391171797No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG29800.17404336015820424No Hit
GTATCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA28770.1680277675084408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28380.1657500188352294No Hit
GTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTG27740.16201217485867736No Hit
GTAGTAAACAGCCGTGTCCTCGGCTCTCAGGCCGTTCATTTGCACATACA26700.1559381783967803No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA26540.1550037174026423No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG26380.1540692564085043No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTTCCAGATCCACTTCCA26120.15255075729303003No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25240.14741122182527097No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG24110.1408115910541713No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC24000.14016914912070141No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23610.13789140044749001No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT23470.13707374707761924No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT22310.1302989048701187No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG21090.12317363978981637No Hit
CTCCAAAGGGGATGCATCGTAGATCAGGAGTTTAGGGGCTTCCCCTGGTT20820.12159673686220848No Hit
GTTTACTACTGTGCGAGAGCAAAAGAAAGGACCCACACAGATTATTGGAG20480.1196110072496652No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20440.11937739200113069No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGGA20380.11902696912832894No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTTCCAGATCCACTTCCA20280.11844293100699269No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT20070.11721645095218655No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19550.11417945272123801No Hit
GTTTAGGGGCTTCCCCTGGTTTCTGCTGATACCAATTTAAATAGTTGCTA18740.10944874393841436No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18700.10921512868987986No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18320.10699578382880207No Hit
ACATATTACTGTCAACACTATGATAATCTCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAGCGAA18270.10670376476813394No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG17950.10483484277985793No Hit
CCCCTAAACTCCTGATCTACGATGCATCCCCTTTGGAGACAGGGGTCCCA17840.10419240084638805No Hit
ACCCAGTATCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCAT17780.1038419779735863No Hit
ATATTACTGTCAACACTATGATAATCTCTTATTCACTTTCGGCCCTGGGA17600.10279070935518105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7400.046.864021
GTATCAA62700.040.5241621
TATCAAC71100.036.0262532
ATCAACG76300.033.6609953
TCAACGC77450.033.249854
CAACGCA78600.032.8507425
AACGCAG79800.032.3997736
CGCAGAG85900.030.0937028
AGAGTAC86600.029.6522411
ACGCAGA87800.029.4424727
CAGAGTA87900.029.2918110
GCAGAGT89100.028.9358379
TCTGGAG15150.027.88794570
CCGTTCA9800.025.99933632
AGTACGG68250.025.85438713
GAGTACG69500.025.6363612
CGTTGTC10250.025.480363
CGTAGAG2050.025.16212828
GCCGTTC10300.025.06491331
ACGTAGA2200.025.00231727
Produced by FastQC (version 0.11.5)