FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712217
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42690.24932587399844763No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG39070.22818369400607516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30240.17661312789208378No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29200.17053913143018673No Hit
GTATCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA28600.16703490270216917No Hit
GTGCAGCCACAGTTCGCTTGAGATCCACTTTGGTCCCAGGGCCGAAAGTG27360.1597928299975996No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAA26940.15733986988798732No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC26620.1554709478997113No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26090.15237554585662916No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24940.14565910746126223No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24100.14075318724203767No Hit
GTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTTCCAGATCCACTTCCA23730.13859224619309352No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23230.13567205558641224No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC23170.13532163271361047No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT22770.13298548022826548No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22190.12959805912451516No Hit
GTAGTAAACAGCCGTGTCCTCGGCTCTCAGGCCGTTCATTTGCACATACA21700.12673627232996754No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21670.12656106089356664No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21390.12492575415382512No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT21000.12264800548061373No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT20750.1211879101772731No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20660.12066227586807048No Hit
CTCCAAAGGGGATGCATCGTAGATCAGGAGTTTAGGGGCTTCCCCTGGTT20090.1173332585764538No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20080.11727485476432017No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19980.11669081664298392No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19450.11359541459990176No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19220.11225212692082837No Hit
AGTCAGGACATTAGCAACTATTTAAATTGGTATCAGCAGAAACCAGGGGA19060.11131766592669037No Hit
ATATTACTGTCAACACTATGATAATCTCTTATTCACTTTCGGCCCTGGGA18270.10670376476813394No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18190.10623653427106494No Hit
CAGTAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAATCTGTTCCAGATCCACTTCCA18180.1061781304589313No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18170.10611972664679768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18160.10606132283466406No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18030.10530207327692694No Hit
ACATATTACTGTCAACACTATGATAATCTCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATCTCAAGCGAA17930.10471803515559068No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17810.10401718940998717No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG17560.10255709410664654No Hit
GTTTACTACTGTGCGAGAGCAAAAGAAAGGACCCACACAGATTATTGGAG17470.10203145979744391No Hit
ACCCAGTATCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCAT17410.10168103692464213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7700.053.955481
GTATCAA69050.042.1670341
TATCAAC76850.037.7311132
ATCAACG81700.035.385523
TCAACGC82500.034.8526844
CAACGCA84400.034.1393245
AACGCAG85250.033.7587436
CGCAGAG91500.031.190718
AGAGTAC92900.030.64601311
GCAGAGT94150.030.4583599
ACGCAGA94500.030.418067
CAGAGTA93600.030.38110710
TGCGTAG1009.928047E-525.73283869
AGTACGG71500.025.6868513
GCGTTGT8400.025.60848862
GCGTAGG1300.001068554825.59745670
GAGTACG72150.025.59417312
AGTACTT39600.025.17966313
GAGTACT39650.025.14423612
CCGTTCA8200.024.33437332
Produced by FastQC (version 0.11.5)