FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417841
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5080.12157734640688683No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG401.4265717E-462.14562670
TTCGGTA306.448134E-445.79360213
GTGGTAT1950.042.2760731
GGTATCA7600.041.5807461
GTATCAA18200.039.2563551
TCGGTAT350.001376938239.2516614
TCTCGTA901.200533E-1038.40955438
TCGTATG852.8649083E-936.65060440
ACGGACT1350.035.6513827
CACGGAT400.002652297734.34527
TCAACGC21250.033.94524
TATCAAC21150.033.943292
ATCAACG21350.033.625323
CAACGCA21550.033.4726455
ACGAGAC1450.033.17279422
CTCGTAT958.54925E-932.76893239
GACGGAC1600.032.21790326
CGAGACG1500.032.0670323
AACGCAG22800.031.7881776
TGGTATC2600.031.7070542
Produced by FastQC (version 0.11.5)