FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343615
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT48570.3614874796723764No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA41250.30700758773904724No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA35910.26726406001719244No Hit
GTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA32970.2453827919456094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC31140.23176281896227716No Hit
GTGTGTACCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC30910.2300510190791261No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG30810.22930675826036478No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30760.2289346278509841No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT28800.2143471158032621No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26560.19767567346300838No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC26510.19730354305362768No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26070.19402879545107787No Hit
TTATTACTGCATGCAAGGTACACACTGGCCCTGGACGTTCGGCCAAGGGA25520.18993536094789057No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25350.18867011755599633No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24530.18256717884215345No Hit
GGTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT23960.17832489217521388No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCGCTGATTTCACACTGCA23890.17780390960208095No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC23600.1756455532276731No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC22880.17028687533259154No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG20590.15324330258295718No Hit
GATCAGCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAGCTGGG20150.14996855498040734No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC19590.1458006943953439No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAATAGCTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG19350.14401446843031673No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19220.143046929365927No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG19180.14274922503842247No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19150.14252594679279407No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18890.14059086866401463No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGATA18790.1398466078452533No Hit
AGGTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18690.13910234702649196No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18600.13843251228960676No Hit
GGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC18560.13813480796210223No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT18480.1375393993070932No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18230.13567874726018986No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC17980.13381809521328655No Hit
GTGTATTACTGTGCGATAAGTCCTCTCGCTGCGCGTGACTACTGGGGCCA17920.13337153872202975No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT17700.13173416492075482No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17230.12823613907257658No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG17120.12741745217193914No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16910.12585450445254034No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAG16310.12138893953997239No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATAGCTAT16250.12094238304871559No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC16160.1202725483118304No Hit
AGTGTGTACCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTGCAGTGTGAAATCAG16120.11997484398432587No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16090.11975156573869747No Hit
GGTCTAGTCAAAGCCTCGTAAACCGTGATGGAAACACTTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCTC16070.11960271357494519No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16050.11945386141119295No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15990.11900730491993614No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15520.1155092790717579No Hit
GCAGAGGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGACTCTGGGGTCCCAGACAGATT15510.11543485298988176No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15320.11402075743423526No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15160.11282994012421714No Hit
CACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT15100.11238338363296033No Hit
CAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTGCAGTGTGAAATCAGCGCCTGACCCACTGCCG15000.11163912281419901No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14960.11134141848669447No Hit
GCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAGCTGGGTTTTC14900.11089486199543769No Hit
GATTTCACACTGCAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAGGTACACACTGG14720.1095551925216673No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACT14570.1084388012935253No Hit
AGTCAAAGCCTCGTAAACCGTGATGGAAACACTTACTTGAATTGGTATCAGCAGAGGCCAGGCCAATCTCCAAGG14520.10806667088414464No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTG14480.1077689665566401No Hit
GGGCTGGAGTGGGTGGCCAAGATAAAGGACGATGGAAGTGAAAAATACTA14350.10680142749225037No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT14340.10672700141037424No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTAAA14210.10575946234598452No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14160.10538733193660386No Hit
CTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCATT14030.10441979287221415No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA13870.10322897556219603No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT13830.10293127123469148No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13710.1020381582521779No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13560.10092176702403591No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13470.1002519322871507No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGA13450.10010308012339846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT4200.047.6188281
GTATCAA34350.038.9497761
CGTATGC601.306675E-534.6373241
TATCAAC39100.034.1285632
ATCAACG41800.031.8415973
TCAACGC42400.031.3910064
TCTGGAG18000.031.1635670
CAACGCA43000.031.0331845
AACGCAG43350.030.7826256
CGCAGAG46250.029.0685628
CTTATAC953.2186654E-729.0379751
AGAGTAC47000.028.38466511
CAGAGTA47900.027.9233110
GCAGAGT48300.027.8348039
GCGTTAA500.00744939927.81385652
CGGCAAT500.007751625527.58813324
ACGCAGA49300.027.2002777
TGCACAG10100.027.12370970
ATCAGCG16400.026.64723470
TCGTATG658.48892E-426.62816240
Produced by FastQC (version 0.11.5)