FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1343615
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45060.33536392493385386No Hit
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA34570.2572909650457907No Hit
GTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA32500.24188476609743118No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA31410.2337723231729327No Hit
GTGTGTACCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCC30090.22394808036528321No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT28360.2110723682007123No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27620.20556483814187843No Hit
TTATTACTGCATGCAAGGTACACACTGGCCCTGGACGTTCGGCCAAGGGA25580.19038191743914737No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC25260.18800028281911113No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25100.186809465509093No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC23910.17795276176583322No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23800.17713407486519575No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23440.17445473591765498No Hit
GGTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT21510.1600905021155614No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21350.15889968480554326No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC21340.15882525872366712No Hit
GTGTATTACTGTGCGATAAGTCCTCTCGCTGCGCGTGACTACTGGGGCCA21320.15867640655991486No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCGCTGATTTCACACTGCA21130.15726231100426832No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20860.15525280679361275No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT20700.15406198948359462No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20070.14937314632539828No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19630.14609839872284844No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAATAGCTATTGGATGAACTGGGTCCGCCAGGCTCCAGGGAAGGGG18900.14066529474589076No Hit
AGGTACACACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT18890.14059086866401463No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18650.13880464269898746No Hit
GATCAGCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAGCTGGG18480.1375393993070932No Hit
GGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC18170.13523219076893306No Hit
GTCTGGGACCCCAGAGTCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGATA18000.13396694737703882No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17920.13337153872202975No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17790.13240399965764005No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16970.12630106094379714No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG16940.12607778269816874No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16780.12488696538815064No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16610.12362172199625637No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC16550.12317516550499957No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATCCAATAGCTAT16120.11997484398432587No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGAC15950.1187096005924316No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15850.11796533977367028No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15640.11640239205427148No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15310.11394633135235913No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15240.11342534877922619No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15170.11290436620609327No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15130.11260666187858873No Hit
AGTGTGTACCTTGCATGCAGTAATAAAGCCCAACATCCTCAGCCTCCACCCTGCTGATTTGCAGTGTGAAATCAG15100.11238338363296033No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGT14990.11156469673232287No Hit
GCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGC14990.11156469673232287No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG14820.11029945334042862No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCATTATGGAGTTGGGGCTGAG14220.10583388842786065No Hit
GATTTCACACTGCAAATCAGCAGGGTGGAGGCTGAGGATGTTGGGCTTTATTACTGCATGCAAGGTACACACTGG14130.10516405369097546No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14050.1045686450359664No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTGATGACTCAGTCTCCACTCTCC13940.10374995813532895No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC13880.10330340164407216No Hit
CACTGGCCCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCAT13760.10241028866155855No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA13600.10121947135154044No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13590.10114504526966431No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13560.10092176702403591No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA13520.10062406269653137No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG150.00228934769.4984252
GTGGTAT4350.051.573621
GTATCAA34400.041.337321
TATCAAC40300.035.2657282
ATCAACG42450.033.2172433
TCAACGC43250.032.5821844
CAACGCA43550.032.3481065
AACGCAG44200.031.9503256
ATTCGTG450.00443519930.92766855
CGCAGAG47200.030.0655338
AGAGTAC47800.029.61608111
TCTGGAG18500.029.43855770
GCAGAGT47800.029.3999049
CAGAGTA48450.029.36094310
ACGCAGA49500.028.7381327
GGTACCG756.232842E-527.5552066
TTCGGGG12850.025.842970
GAGTACG39850.025.32426312
AGTACGG39850.025.2387713
GCTTGTA1951.8189894E-1224.7797551
Produced by FastQC (version 0.11.5)