FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479561
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12970.2704556875976153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10100.042.65081
GTGGTAT3000.042.502941
TGATCGG350.001233432640.14131559
GTATCAA22900.038.2240561
TGGTATC3450.035.922582
CGAATTA701.0097665E-634.33246615
TATCAAC26150.033.8333932
ATCAACG26500.033.355153
TCAACGC27000.032.699884
CAACGCA27700.031.8469455
AACGCAG28250.031.3484216
CGCTAAG802.301571E-630.89470763
GATATAA802.7810784E-630.1541141
ACGCAGA30200.029.4379277
CGCAGAG31300.028.4033688
AGAGTAC32100.027.69549411
GCAGAGT32450.027.5025549
ACGGGGC2750.027.46883616
CAGAGTA32500.027.35462610
AGTACGG22000.026.83614513
Produced by FastQC (version 0.11.5)