FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041143
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT52300.5023325326107941No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT42010.4034988469403338No Hit
CTGTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC36630.3518248693983439No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA29630.2845910696225207No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29250.28094123477754734No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28510.2738336616583889No Hit
GGTCTATGGTGCCTCCCTTTTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA28400.27277713051905456No Hit
GTCTATTACTGTGCGAGGCAGTGGGAACTAAAGACATGGTTCGACCCCTGGGGCGAGGGAATCCTGGTCACCGT27520.26432488140437965No Hit
ACCTACTACTACGCGTCCCTCAAGAGCCGGCTCACCATTTCCGTCCACGCGTCTAGGAATCAGGTCTCCCTGAAA26760.2570252117144331No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26100.2506860248784269No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25470.24463498289860278No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT25230.242329824049146No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAACCAATTCTTTAGCCTGGTATCAGCAGAAA24130.23176451265580233No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22130.21255485557699566No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21680.20823268273426418No Hit
CCATAGACCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC21260.2041986547477148No Hit
ACCTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAA20090.19296100535661287No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19200.1844127079565439No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC19090.18335617681720953No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT18680.1794181971160542No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18500.1776893279789616No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC18090.17375134827780622No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTA17660.16962127200586277No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.1684686925811344No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG17250.16568329230470744No Hit
GTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCCCGATCCACTGCCA16780.16116902289118787No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16690.16030458832264155No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16540.15886386404173106No Hit
CAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT16500.1584796709001549No Hit
GTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCC16420.15771128461700265No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC16110.15473378776978763No Hit
AGTCAGGGCATAACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAA16100.1546377394843936No Hit
CTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGA15520.14906693893153966No Hit
CTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAACC15420.14810645607759934No Hit
GGGCATAACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGTCTCCTGGTCTATGGTGCCTC15110.1451289592303843No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCGTCACTT15100.14503291094499027No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15040.14445662123262606No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14950.14359218666407977No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC14850.14263170381013943No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14760.14176726924159314No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14550.13975025524831844No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14240.1367727584011034No Hit
GATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCAACAGTATTACAGTACC14230.13667671011570937No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG14120.13562017897637502No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTAAGAGGGGTA13890.13341106841231223No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCTGTCAGGACACAGCATGGA13680.13139405441903754No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT13550.1301454267089151No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13480.12947308871115687No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13410.12880075071339864No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA13190.1266876884347299No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13020.12505486758303133No Hit
GGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCAACAGTATTACAGTAC13000.12486277101224329No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT12620.12121293616727002No Hit
CAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCCCGATCCACTGCCA12610.12111688788187598No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12190.11708285989532657No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12080.11602632875599223No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGG12010.11535399075823399No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11930.11458560447508172No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC11890.11420141133350557No Hit
CTCTTATAGTGGGACGACCTACTACTACGCGTCCCTCAAGAGCCGGCTCA11860.11391326647732349No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA11610.11151205934247264No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11600.11141601105707861No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11530.11074367305932038No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11520.11064762477392634No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA11490.11035947991774424No Hit
TTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCATCTGTCTT11230.10786222449749938No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11220.10776617621210535No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG11180.10738198307052922No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11110.10670964507277098No Hit
GATTACTACTGGGCCTGGATCCGACAGTCCCCAGGGAAGGGCCTGGAGTGGATTGGCA10980.10546101736264854No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10920.10488472765028435No Hit
CTCCTGGTCTATGGTGCCTCCCTTTTGGAAAGTGGGGTCCCATCCAGGTT10720.1029637619424037No Hit
GTGTAATCCGACCCCGATCCACTGCCACTGAACCTGGATGGGACCCCACT10660.10238747223003948No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC10580.1016190859468872No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10510.10094674794912897No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAA6350.058.41926670
CGCGTAT252.5381887E-455.32653839
TCGCGTA200.007312095351.83344338
GTGGTAT3850.048.9383351
GTATCAA31150.043.8795361
CTTATAC2650.041.3667451
GCGTGAA1050.039.9803556
TATACAC2950.038.3230443
TATCAAC35900.038.075582
GGACGGA6850.036.47097469
TGCCGTC1051.4551915E-1136.35616345
ATGCCGT1151.8189894E-1236.1966144
ATCAACG38550.035.5470623
TTATACA3300.035.2966162
TCAACGC38800.035.2297254
TGCGTGA904.5638444E-934.95708555
ACTCGAC3150.034.92305827
CAACGCA39400.034.780185
AACGCAG40350.033.961326
CTCTTAT3250.033.7298051
Produced by FastQC (version 0.11.5)