FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1041143
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT53370.5126096991479556No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40120.3853457210008615No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34700.3332875503172955No Hit
CTGTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGAC32340.3106201549643037No Hit
GTAATAGACAGCCGTGTCTGCGGCGGTCACAGAGCTCAGTTTCAGGGAGA28720.2758506756516636No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26580.2552963425773405No Hit
ACCTACTACTACGCGTCCCTCAAGAGCCGGCTCACCATTTCCGTCCACGCGTCTAGGAATCAGGTCTCCCTGAAA25550.24540336918175507No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25260.24261796890532808No Hit
GGTCTATGGTGCCTCCCTTTTGGAAAGTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACA24990.24002466519968918No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24760.23781555463562642No Hit
GTCTATTACTGTGCGAGGCAGTGGGAACTAAAGACATGGTTCGACCCCTGGGGCGAGGGAATCCTGGTCACCGT24170.23214870579737848No Hit
GTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAACCAATTCTTTAGCCTGGTATCAGCAGAAA23130.22215968411639897No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22230.21351533843093598No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC20400.19593850220382789No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20230.19430568135212933No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20030.1923847156442487No Hit
CAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTACCAGATGTGACAT19950.1916163293610964No Hit
ACCTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAA19740.1895993153678217No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18860.18114706625314678No Hit
TGTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTC18620.17884190740368996No Hit
GTATCAACGCAGAGTACGGGGAGTCAGACCCTGTCAGGACACAGCATGGA17650.16952522372046874No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17270.1658753888754955No Hit
GTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTA17080.16405047145300886No Hit
GTCAGGACACAGCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCC17070.16395442316761483No Hit
GTCTCCATCCTCCCTGTCTGCCTCTGTAGGAGACAGAGTCACCGTCACTT16700.1604006366080356No Hit
CCATAGACCAGGAGACTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGC16370.1572310431900325No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16150.15511798091136375No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15980.1534851600596652No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15690.15069975978323824No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15600.14983532521469192No Hit
CAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCAT15570.14954718035850983No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15300.14695387665287094No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG15050.14455266951802012No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14800.14215146238316928No Hit
CTGTAGGAGACAGAGTCACCGTCACTTGCCGGGCGAGTCAGGGCATAACC14740.14157517267080508No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14520.13946211039213635No Hit
GTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGGCCTGGATCCG14520.13946211039213635No Hit
GCATGGACATGGGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14440.1386937241089841No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14270.1370609032572855No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14240.1367727584011034No Hit
GTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCCCGATCCACTGCCA14090.1353320341201929No Hit
GGGCATAACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAGTCTCCTGGTCTATGGTGCCTC13800.13254663384376594No Hit
GTAATACTGTTGACAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCC13790.1324505855583719No Hit
CTATTACTGTCAACAGTATTACAGTACCCCTCTTACTTTCGGCGGAGGGA13650.13110590956285545No Hit
AGTCAGGGCATAACCAATTCTTTAGCCTGGTATCAGCAGAAACCAGGGAA13580.13043357156509722No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13520.12985728185273301No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13370.12841655757182252No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTATGATCTCCAGCTTGGTCCCTCCGCCGAAAGTAAGAGGGGTA13200.12678373672012394No Hit
GGATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCAACAGTATTACAGTAC13000.12486277101224329No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12870.12361414330212084No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12790.12284575701896856No Hit
GATTACACTCTCACCATCAGCAGCCTGCAGCCTGAAGATTTTGCAACCTATTACTGTCAACAGTATTACAGTACC12660.12159712930884614No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC12650.12150108102345211No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12470.11977221188635952No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11990.11516189418744592No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11700.11237649391101895No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT11500.11045552820313828No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11300.10853456249525761No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG11280.10834246592446954No Hit
TTACAGTACCCCTCTTACTTTCGGCGGAGGGACCAAGCTGGAGATCATACGAACTGTGGCTGCACCATCTGTCTT11210.10767012792671131No Hit
CTCTTATAGTGGGACGACCTACTACTACGCGTCCCTCAAGAGCCGGCTCA10990.10555706564804258No Hit
GTGGGGTCCCATCCAGGTTCAGTGGCAGTGGATCGGGGTCGGATTACACT10910.10478867936489031No Hit
CTCCTGGTCTATGGTGCCTCCCTTTTGGAAAGTGGGGTCCCATCCAGGTT10790.10363609994016193No Hit
GTAGTAGGTCGTCCCACTATAAGAGACACTGCCAATCCACTCCAGGCCCTTCCCTGGGGACTGTCGGATCCAGGC10760.10334795508397981No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10750.10325190679858579No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTACTGATTACTACTGGG10620.10200327908846335No Hit
CAGTAATAGGTTGCAAAATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGTAATCCGACCCCGATCCACTGCCA10470.10056255480755286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAA6100.058.35043770
TAGTACG309.623585E-656.986484
GTGGTAT3800.052.2881051
TTACGGC200.007624513551.2878344
GTATCAA35350.041.6713521
GGACGGA6650.040.46544369
TATCAAC38650.037.8238072
CTTATAG2550.036.2345123
ATCAACG42000.034.873323
CCTACTA6700.034.747292
TCAACGC42600.034.191894
ACCTACT6900.033.761241
CAACGCA43550.033.5180975
TACGCGT7300.033.24873410
AACGCAG44350.033.067656
CTACGCG7350.033.0225569
CGCGTCC7500.032.35588512
CTCTTAT3300.032.18151
ACTACGC7550.032.1477858
GCGTCCC7450.032.12043813
Produced by FastQC (version 0.11.5)