FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722185
Sequences flagged as poor quality0
Sequence length20-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31980.18569433597435814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76750.039.7735441
GGTATCA33400.039.1108131
GTGGTAT12300.037.7301371
TATCAAC86850.035.1297722
ATCAACG92400.032.8375323
TGGTATC13750.032.734132
TCAACGC94100.032.262954
CAACGCA94350.032.2016645
AACGCAG96950.031.476826
ACGCAGA103800.029.3335277
CGCAGAG104950.028.914098
AGAGTAC104850.028.84355711
CAGAGTA107250.028.19810710
GCAGAGT110200.027.6921759
GCGTTAG500.00787269427.5010781
AGTACGG68000.024.85989413
GAGTACG69050.024.57975412
GAGTACT56850.023.22021912
AGTACTT56950.022.93995313
CTATACG800.002961055821.4852161
Produced by FastQC (version 0.11.5)