FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1066855
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33000.3093203856194141No Hit
GTAATAGACGGCCGTGTCTGCGGCGGTCACAAAGCGCAATGTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA28070.2631097946768774No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22280.20883812701819834No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19660.1842799630690206No Hit
CTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGG19480.182592760965642No Hit
GTCTATTACTGTGCGAGAGATCGCACGTTCAGGGAGTACCCAAGAACCTA19070.17874968950794626No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG19030.17837475570719546No Hit
GTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA18560.1739692835483735No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18380.17228208144499485No Hit
CAGTAATAGACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA18280.17134474694311785No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18170.17031367899105312No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17360.16272126952584934No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17250.16169020157378464No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16840.15784713011608886No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT16790.15737846286515036No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA16680.15634739491308564No Hit
GTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATT16380.15353539140745462No Hit
GTCTACAAAGTCCCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA15950.14950485304938346No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAA15170.14219364393474276No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAGCCTGACCTGCCTGGTCAAAGGCTT15070.14125630943286577No Hit
GTCTATTACTGCATGCAAGGTCTACAAAGTCCCACTTTCGGCGGAGGGAC14740.13816310557667164No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG14560.136475903473293No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14490.1358197693219791No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC14030.13150803061334485No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13920.13047696266128012No Hit
GTATGTACAGGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13350.12513415600058114No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13290.12457175529945494No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGAC13080.12260335284551321No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGTAGGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGG13040.12222841904476242No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12760.11960388243950679No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTTTTCCTCAT12620.11829161413687896No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12300.11529214373087251No Hit
CTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT12190.1142610757788078No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAG11860.11116787192261365No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG11470.1075122673652933No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGATTCTAATCGGGCC11400.10685613321397942No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11060.10366919590759757No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10930.10245066105515745No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10900.10216946070459436No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10890.10207572725440665No Hit
GGTCTAGTCAGAGCCTCCTGCATAGTGATGGATACAACGATTTGGCTTGGTACCTGCAGAAGCCAGGGCAGTCTC10880.10198199380421895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)