FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193528
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.5689099251787855No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.28161299656897193No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC3340.1725848456037369No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT2280.11781240957380844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2100.10851142987061305No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.10489438220825927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.10076061345128354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATAGG350.006715510552.9279670
AAAGGCG350.006715510552.9279670
GCCGTCT1300.048.83010546
CTCGTCC304.8545023E-448.4930661
CCGTCTT1400.048.01736547
TATACAC2200.047.4192963
GTGGTAT2700.046.1162571
CTTATAC2300.045.357591
TTATACA2400.042.0655062
CCCTAAG2350.041.72265628
CGAGACC2350.041.5607323
ACGAGAC2350.041.5499822
TCGTATG1500.041.509740
GCAATAG600.001085752641.1661970
CGTATGC1550.040.35297841
TGCCGTC1850.039.90216445
AGACCCT2450.039.87469525
CCCACGA2450.039.85406519
TACACAT2550.039.5910645
CGTCTTC1650.038.68180548
Produced by FastQC (version 0.11.5)