FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193528
Sequences flagged as poor quality0
Sequence length20-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20861.0778802033814228No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5410.2795461121904841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3180.16431730808978545No Hit
CTATAGGGAAGTGATACCAGCATCGTCTTGATGCCCTTGGCAGCACTTCC3030.15656649167045594No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCCGG250.006974729752.43409769
GTGGTAT2700.046.0054971
GGTATCA9600.040.215951
CACTCAC350.001242527640.0685944
GTATCAA22600.039.3649021
TGGTATC3200.037.7387122
TCCCGGC400.002540360434.63862240
TATCAAC25850.034.3890272
ACGGGGC1800.033.5281816
TTAACTG400.002988176233.5108535
TCAGTTG450.00404227631.50514848
TCAACGC28650.031.1210844
ATCAACG28750.031.0288773
CAACGCA29050.030.6846355
CGGGGCT1101.4169927E-930.48804517
AACGCAG29450.030.3816536
CCAGCGG450.00525831129.85685724
CATAAAC500.00610639428.9560758
GCCATAA500.006249317428.81933256
ACGCAGA32050.027.9169987
Produced by FastQC (version 0.11.5)