FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425973
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTC74810.5246242390283687No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT65520.45947574042425765No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC52680.36943195979166504No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG51210.35912320920522334No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47420.3325448658565064No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAA46040.32286726326515297No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT45920.3220257326050353No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44880.31473246688401535No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG44220.310104048253368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43280.30351205808244613No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG42450.2976914710166321No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT41700.2924319043908966No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT41100.2882242510903082No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT39810.279177796494043No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC36130.25337085625043393No Hit
GCTTAATAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAAACGAACTGTGGCTGCACCAT36100.2531604735854045No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34440.24151929945377648No Hit
TATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCA34290.24046738612862936No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGCTTGGTCCCCTGGCCAAAAGTG34040.2387141972533842No Hit
CTTTTGGCCAGGGGACCAAGCTGGATATCAAACGAACTGTGGCTGCACCA32500.22791455378187386No Hit
GTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTACAACCTGAAGATT32450.2275639160068248No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC32100.22510945158148157No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31930.22391728314631482No Hit
GCTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGCTA31650.22195371160604024No Hit
GTATCAACGCAGAGTACGGGAAACTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTG29390.20610488417382375No Hit
GCATAGATAAGGAGCTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAGGC29040.20365041974848053No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28710.2013362104331569No Hit
GTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCACAATCAGCAGCC28400.19916225622785286No Hit
GCCCCTAAGCTCCTTATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGT28380.19902200111783322No Hit
GATAAGGAGCTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAGGCTAAAA28260.19818047045771556No Hit
GCCTACAACCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTAATAGTTTCCCGTACACTTTTGGCCAGGGGA27800.19495460292726438No Hit
GGCCAAAAGTGTACGGGAAACTATTAAGCTGTTGACAGGAATAAGTTGCA27230.1909573322917054No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT27030.18955478119150923No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTC26970.1891340158614504No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG26800.18794184742628367No Hit
GAATTCACTCTCACAATCAGCAGCCTACAACCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTAATAGTTTC26490.18576789322097964No Hit
GTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGACAGCCCCTAAGCTCCTTATCTATGCTG26340.18471597989583252No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC24120.1691476626836553No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23380.16395822361292955No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23310.1634673307278609No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT22320.15652470278188998No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT22200.1556831721217723No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22010.15435074857658596No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21660.1518962841512427No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC21490.15070411571607598No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20970.147057482855566No Hit
GTGATGGGACGCCACTTTTCAAAGTGGATGCAGCATAGATAAGGAGCTTA20840.1461458246404385No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA20340.14263944688994812No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20120.14109664067973238No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19960.13997459979957544No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGCTTGGTCC19880.13941357935949697No Hit
ACTTATTCCTGTCAACAGCTTAATAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAAACGAA19670.13794090070429105No Hit
CCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG19650.1378006455942714No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19620.137590262929242No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA19340.13562669138896738No Hit
CCTTATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATT19060.13366311984869278No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC18860.13226056874849665No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18680.1309982727583201No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18230.12784253278287877No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18180.12749189500782973No Hit
GTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCA18060.12665036434771204No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA18020.12636985412767282No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17830.12503743058248648No Hit
TCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCACA17780.12468679280743745No Hit
GCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTCCTGTCTGCATCTGTAGGAGA17650.12377513459230995No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC17430.1222323283820942No Hit
ATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCA16950.11886620574162343No Hit
AGTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGACAGCCCCTAAGCTCCTTATCTATG16800.11781429241647633No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16740.1173935270864175No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT16640.11669225153631942No Hit
GTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGCTAATGTCTTGACTGGCCCGGCAAGTGATGGTGACT16640.11669225153631942No Hit
CCCCTAAGCTCCTTATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCA16550.11606110354123116No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCCCAGTCAGGACACAGCATGGAC16540.11599097598622132No Hit
GAGTCACCATCACTTGCCGGGCCAGTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGACAG16210.1136767666708977No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAGACATTAGC15790.11073140936048578No Hit
GGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGACCCACTGCCG15740.11038077158543676No Hit
GGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTC15520.10883796537522099No Hit
CTCACAATCAGCAGCCTACAACCTGAAGATTTTGCAACTTATTCCTGTCA15330.10750554183003465No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAG15250.10694452138995619No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT15070.10568222539977966No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15050.10554197028976005No Hit
CACCATCACTTGCCGGGCCAGTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGACAGCCC14940.10477056718465216No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC14680.10294725075439717No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTC14510.10175508231923046No Hit
TGTCTTGACTGGCCCGGCAAGTGATGGTGACTCTGTCTCCTACAGATGCA14450.1013343169891716No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT14360.10070316899408334No Hit
TCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGC14350.10063304143907352No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14300.1002824036640245No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGTA150.0022687369.6573551
GTGGTAT6350.057.856081
TAATGAT5700.041.77226370
CGTATGC503.7615373E-641.52992641
GTATCAA58400.039.5677341
TCGTATG655.3423537E-737.2506940
TATCAAC65250.035.308642
ATCAACG70150.032.940213
TCAACGC70550.032.753454
CAACGCA71700.032.2281155
AACGCAG72450.031.894496
AATGGTA2950.031.38819970
CGCAGAG77000.029.912248
AGAGTAC77950.029.32751311
CAGAGTA78200.029.27764710
ACGCAGA79000.029.2418677
GCAGAGT79300.029.0013859
ACGAGAC1005.227266E-727.47197522
CTTATAC1155.909351E-826.8709871
GACAGGT3250.026.4116541
Produced by FastQC (version 0.11.5)