FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425973
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTC90430.6341634799536877No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT61120.4286196162199425No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG44630.3129792780087702No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC42260.2963590474714458No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41210.288995654195416No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39550.27735448006378804No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39420.27644282184866054No Hit
GTATCAACGCAGAGTACGGGAAACTATTAAGCTGTTGACAGGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTG37200.2608745046364833No Hit
GTCTCCATCCTTCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT36510.2560357033408066No Hit
GTGCAGCCACAGTTCGTTTGATATCCAGCTTGGTCCCCTGGCCAAAAGTG36260.25428251446556144No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT35990.2523890704802966No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35470.24874243761978665No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33660.2360493501630115No Hit
CTTTTGGCCAGGGGACCAAGCTGGATATCAAACGAACTGTGGCTGCACCA33430.23443641639778592No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAA33270.233314375517629No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG33230.23303386529758977No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT33000.2314209315323642No Hit
GCTTAATAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAAACGAACTGTGGCTGCACCAT32750.22966774265711903No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC31450.22055116050584406No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31110.21816682363551063No Hit
GTCTGGGACAGAATTCACTCTCACAATCAGCAGCCTACAACCTGAAGATT30840.21627337965024582No Hit
TATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCA30740.21557210410014777No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC29830.2091904965942553No Hit
GGCCAAAAGTGTACGGGAAACTATTAAGCTGTTGACAGGAATAAGTTGCA29710.20834896593413763No Hit
GTCCCAGACCCACTGCCGCTGAACCGTGATGGGACGCCACTTTTCAAAGT28110.19712855713256844No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28100.19705842957755865No Hit
GCTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGCTA27830.1951649855922938No Hit
GTATCAACGCAGAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCT27430.19235988339190152No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25960.18205113280545987No Hit
GTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCACAATCAGCAGCC25850.18127972970035197No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25830.18113947459033236No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25360.17784347950487142No Hit
GCCTACAACCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTAATAGTTTCCCGTACACTTTTGGCCAGGGGA25210.1767915661797243No Hit
GAATTCACTCTCACAATCAGCAGCCTACAACCTGAAGATTTTGCAACTTATTCCTGTCAACAGCTTAATAGTTTC23960.1680256218034984No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23870.1673944738084101No Hit
GCATAGATAAGGAGCTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAGGC23830.16711396358837088No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23020.16143363163257649No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA23000.16129337652255687No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22900.1605921009724588No Hit
GCCCCTAAGCTCCTTATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGT22820.16003108053238035No Hit
GATAAGGAGCTTAGGGGCTGTCCCTGGTTTTTGCTGATACCAGGCTAAAA22390.15701559566695864No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTCTCCATCCTTC22280.15624419256185076No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22240.15596368234181152No Hit
GGTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGACCCACTGCC22100.15498189657167422No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT21730.15238717703631136No Hit
GGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGACCCACTGCCG20850.1462159521954483No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20600.1444627633202031No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20560.1441822531001639No Hit
GAGTACGGGAGTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCT20530.14397187043513446No Hit
GTCAAGACATTAGCAGTTTTTTAGCCTGGTATCAGCAAAAACCAGGGACAGCCCCTAAGCTCCTTATCTATGCTG20060.14067587534967352No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19920.13969408957953622No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19430.13625783938405567No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19130.13415401273376143No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18340.12861393588798664No Hit
GTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGACCCACTGCCG18280.1281931705579278No Hit
GTGATGGGACGCCACTTTTCAAAGTGGATGCAGCATAGATAAGGAGCTTA18230.12784253278287877No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCAGCTTGGTCC17720.1242660274773786No Hit
ACTTATTCCTGTCAACAGCTTAATAGTTTCCCGTACACTTTTGGCCAGGGGACCAAGCTGGATATCAAACGAA17670.12391538970232957No Hit
CCTTATCTATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATT17360.12174143549702554No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA17300.1213206701669667No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA17100.11991811906677055No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCC17020.1193570986266921No Hit
GTATCAACGCAGAGTACGGGAGTCAGTCCCAGTCAGGACACAGCATGGAC16750.1174636546414273No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16450.11535982799113306No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16350.11465855244103501No Hit
CCGCTGAACCGTGATGGGACGCCACTTTTCAAAGTGGATGCAGCATAGAT16120.11304561867580944No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC15750.11045089914044656No Hit
GTCCCTGGTTTTTGCTGATACCAGGCTAAAAAACTGCTAATGTCTTGACTGGCCCGGCAAGTGATGGTGACT15620.10953924092531907No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15620.10953924092531907No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15510.10876783782021118No Hit
CTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAATTGACCCAGTC15290.10722503160999543No Hit
TCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCACA15270.1070847764999758No Hit
ATGCTGCATCCACTTTGAAAAGTGGCGTCCCATCACGGTTCAGCGGCAGTGGGTCTGGGACAGAATTCACTCTCA15120.10603286317482868No Hit
GTTGACAGGAATAAGTTGCAAAATCTTCAGGTTGTAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGACCCA15010.10526146006972083No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC15000.105191332514711No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA14760.10350827119447563No Hit
GATTGTGAGAGTGAATTCTGTCCCAGACCCACTGCCGCTGAACCGTGATG14750.10343814363946582No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14720.1032277609744364No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14630.10259661297934815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14470.10147457209919122No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC14370.10077329654909314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT7750.056.719761
GTATCAA60350.040.573121
TATCAAC67350.036.292422
ATCAACG70800.034.2550163
TCAACGC71150.034.0203864
CAACGCA72350.033.4858865
AACGCAG73300.033.1921426
TAATGAT5050.032.11269470
CGCAGAG78650.030.8907498
AGAGTAC79600.030.26378311
CAGAGTA80400.030.04789410
ACGCAGA80950.030.0130637
GCAGAGT80550.029.9919389
GCTTGTC2700.029.2543331
AATTCGA500.007608796527.6935741
GAGTACT27450.025.71614312
AGTACTT26900.025.35199713
GAGTACG69750.024.41704612
AGTACGG69700.024.23960513
GACAGGT3300.022.8946951
Produced by FastQC (version 0.11.5)