FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005756517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942979
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACAGCTCCAACAATAAGAACTATCTAGCCTGGTACCAACAAAT61250.3152375810546588No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60890.31338475608846006No Hit
GTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCTCT47400.2439552872161768No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43880.2258387764355662No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39480.20319313795980296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38270.19696558737896808No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG37960.1953700992136302No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37050.19068656943796097No Hit
ATCTAGCCTGGTACCAACAAATACCTGGACAACGTCCTAAGCTCCTCATT33040.17004815800891313No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32850.16907027816564152No Hit
GCTTAGGACGTTGTCCAGGTATTTGTTGGTACCAGGCTAGATAGTTCTTATTGTTGGAGCTGTATAAAAGATT32540.16747479000030366No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG31610.16268832550429005No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT29760.15316686387243506No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAATCTTTTATA29760.15316686387243506No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28300.14565262928729542No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTTCTGTCAGCAATATTATGATATT28140.1448291515245404No Hit
GTATAAAAGATTCTGACTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACAGCCAGGGAGTCTGGAGA27220.14009415438869902No Hit
CCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGACTT26850.138189862062328No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25960.13360926700700318No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25420.13083002955770495No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24970.12851399834995644No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC24950.12841106362961205No Hit
GTAAATGAGGAGCTTAGGACGTTGTCCAGGTATTTGTTGGTACCAGGCTA24860.12794785738806236No Hit
TACCTGGACAACGTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGAA24710.1271758469854795No Hit
GTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGACTTCACTCTCAC24690.12707291226513512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23960.12331579497256533No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG23870.12285258873101562No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAATCTTTTATACAGCTCCAA23020.11847786311637953No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA22710.11688237495104167No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG22680.11672797287052511No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22020.11333112709916063No Hit
CCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGT21890.11266205141692216No Hit
GTTCTGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGC21470.1105004222896902No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21120.10869906468366358No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG21090.10854466260314703No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA20710.10658890291660383No Hit
ATTCAGCGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC20690.10648596819625945No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG20540.10571395779367662No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20430.10514781683178254No Hit
GTCAGAGACCCCGGATTCCCGGGCAGATGCCCAGTAAATGAGGAGCTTAGGACGTTGTCCAGGTATTTGTTGGTA20380.10489048003092158No Hit
ATGATATTCCTTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGA20370.10483901267074941No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAATCTTT20100.1034493939461003No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19760.10169950370024586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.1005672217764577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)