FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942979
Sequences flagged as poor quality0
Sequence length20-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT58410.3006208507657571No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT38500.19814933666292842No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG37740.19423781728984205No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37600.1935172742474314No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC35960.18507662717919235No Hit
GTCCTAAGCTCCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCTCT34960.17992989116197344No Hit
CTTTTATACAGCTCCAACAATAAGAACTATCTAGCCTGGTACCAACAAAT34830.179260815479735No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG33920.17457728570406578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32600.16778359416133679No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31540.16232805398308472No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30880.15893120821172024No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30740.1582106651693096No Hit
GTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACCCCGGATTCCCGGGC30000.1544020805165676No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAATTTATTTCTGTCAGCAATATTATGATATT29770.15321833123260725No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27810.14313072863885817No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25620.13185937676114873No Hit
GAGTGAAGTCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACCCCG24820.1277419879473736No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT24690.12707291226513512No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24380.12547742409979726No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24320.12516861993876413No Hit
GTATCAACGCAGAGTACGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAG24270.1249112831379032No Hit
GCTTAGGACGTTGTCCAGGTATTTGTTGGTACCAGGCTAGATAGTTCTTATTGTTGGAGCTGTATAAAAGATT23840.12269818665049906No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC23410.12048509016309492No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22680.11672797287052511No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC22450.11554422358656476No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22290.11472074582380973No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22240.11446340902294878No Hit
TCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCGCTGCCGCTGAATCGGTCAGAGACC21510.11070629173037896No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG21430.11029455284900144No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21410.11019161812865708No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA20980.10797852164125293No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20920.1076697174802198No Hit
ATCTAGCCTGGTACCAACAAATACCTGGACAACGTCCTAAGCTCCTCATT20700.10653743555643165No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA20390.10494194739109379No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA20290.10442727378937189No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20150.10370673074696123No Hit
GGCTGAAGATGTGGCAATTTATTTCTGTCAGCAATATTATGATATTCCTT20120.10355232866644468No Hit
GTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGACTTCACTCTCAC19900.10242004674265652No Hit
ATATTATGATATTCCTTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT19780.10180243842059022No Hit
CCTCATTTACTGGGCATCTGCCCGGGAATCCGGGGTCTCTGACCGATTCAGCGGCAGCGGGTCTGGGACAGACTT19780.10180243842059022No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGTCAGAATCTTTTATA19670.10123629745869617No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19600.10087602593749083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68400.041.8220671
TATCAAC75950.037.5044822
GGTATCA26850.036.9240071
GTGGTAT15600.034.8652761
ATCAACG81600.034.8412483
TCAACGC82650.034.32964
CAACGCA83650.033.948045
AACGCAG85800.033.2157146
TGGTATC16350.032.823692
ACGCAGA91750.031.1365157
CGCAGAG93800.030.346218
AGAGTAC94800.030.207211
CAGAGTA96600.029.60878810
GCAGAGT96650.029.5224179
AGTACGG66700.025.94723113
GAGTACG68200.025.6766612
GAGTACT44300.025.03522712
AGTACTT44050.025.02334813
TACGGGA23700.023.18953515
TACTTTT49500.022.34452215
Produced by FastQC (version 0.11.5)