FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005756519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005756519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044329
Sequences flagged as poor quality0
Sequence length20-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11120.10647985452860162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.038.8186341
GTATCAA42000.037.2613751
GTGGTAT6500.033.865961
TCGTATG1550.033.5393940
TATCAAC48250.032.3619352
ATCAACG50100.030.9609853
TCAACGC51000.030.4820524
CAACGCA52000.029.9620025
TGGTATC7400.029.7457032
AACGCAG53300.029.295756
CGTATGC1850.028.12364441
ACGCAGA57350.027.0405247
CGCAGAG58100.026.809838
ACGGGTA2250.025.981216
CTCGTAT1607.4578566E-1125.97297339
CAGAGTA61400.025.6489210
AGAGTAC61450.025.5161411
TATGCCG2200.025.27243643
GCAGAGT62050.025.2694079
TATACAC3350.024.6400953
Produced by FastQC (version 0.11.5)